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Volume 22
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10.3390/ijms22073524
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Article

The Mitogen-Activated Protein Kinase PlMAPK2 Is Involved in Zoosporogenesis and Pathogenicity of Peronophythoralitchii

by
Jiamin Huang
,
Pinggen Xi
,
Yizhen Deng
,
Weixiong Huang
,
Jingrui Wang
,
Qingqing Zhao
,
Wensheng Yang
,
Wen Li
,
Junjian Situ
,
Liqun Jiang
,
Tianfang Guan
,
Minhui Li
,
Zide Jiang
and
Guanghui Kong
*
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Department of Plant Pathology, South China Agricultural University, Guangzhou 510642, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(7), 3524; https://doi.org/10.3390/ijms22073524
Submission received: 29 January 2021 / Revised: 21 March 2021 / Accepted: 24 March 2021 / Published: 29 March 2021
(This article belongs to the Section Molecular Genetics and Genomics)
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Abstract

As an evolutionarily conserved pathway, mitogen-activated protein kinase (MAPK) cascades function as the key signal transducers that convey information by protein phosphorylation. Here we identified PlMAPK2 as one of 14 predicted MAPKs encoding genes in the plant pathogenic oomycete Peronophythora litchii. PlMAPK2 is conserved in P.litchii and Phytophthora species. We found that PlMAPK2 was up-regulated in sporangium, zoospore, cyst, cyst germination and early stage of infection. We generated PlMAPK2 knockout mutants using the CRISPR/Cas9 method. Compared with wild-type strain, the PlMAPK2 mutants showed no significant difference in vegetative growth, oospore production and sensitivity to various abiotic stresses. However, the sporangium release was severely impaired. We further found that the cleavage of the cytoplasm into uninucleate zoospores was disrupted in the PlMAPK2 mutants, and this developmental phenotype was accompanied by reduction in the transcription levels of PlMAD1 and PlMYB1 genes. Meanwhile, the PlMAPK2 mutants exhibited lower laccase activity and reduced virulence to lychee leaves. Overall, this study identified a MAPK that is critical for zoosporogenesis by regulating the sporangial cleavage and pathogenicity of P.litchii, likely by regulating laccase activity.
Keywords: MAPK; Peronophythora litchii; zoosporogenesis; sporangial cleavage; pathogenicity; CRISPR/Cas9 MAPK; Peronophythora litchii; zoosporogenesis; sporangial cleavage; pathogenicity; CRISPR/Cas9

Share and Cite

MDPI and ACS Style

Huang, J.; Xi, P.; Deng, Y.; Huang, W.; Wang, J.; Zhao, Q.; Yang, W.; Li, W.; Situ, J.; Jiang, L.; et al. The Mitogen-Activated Protein Kinase PlMAPK2 Is Involved in Zoosporogenesis and Pathogenicity of Peronophythoralitchii. Int. J. Mol. Sci. 2021, 22, 3524. https://doi.org/10.3390/ijms22073524

AMA Style

Huang J, Xi P, Deng Y, Huang W, Wang J, Zhao Q, Yang W, Li W, Situ J, Jiang L, et al. The Mitogen-Activated Protein Kinase PlMAPK2 Is Involved in Zoosporogenesis and Pathogenicity of Peronophythoralitchii. International Journal of Molecular Sciences. 2021; 22(7):3524. https://doi.org/10.3390/ijms22073524

Chicago/Turabian Style

Huang, Jiamin, Pinggen Xi, Yizhen Deng, Weixiong Huang, Jingrui Wang, Qingqing Zhao, Wensheng Yang, Wen Li, Junjian Situ, Liqun Jiang, and et al. 2021. "The Mitogen-Activated Protein Kinase PlMAPK2 Is Involved in Zoosporogenesis and Pathogenicity of Peronophythoralitchii" International Journal of Molecular Sciences 22, no. 7: 3524. https://doi.org/10.3390/ijms22073524

APA Style

Huang, J., Xi, P., Deng, Y., Huang, W., Wang, J., Zhao, Q., Yang, W., Li, W., Situ, J., Jiang, L., Guan, T., Li, M., Jiang, Z., & Kong, G. (2021). The Mitogen-Activated Protein Kinase PlMAPK2 Is Involved in Zoosporogenesis and Pathogenicity of Peronophythoralitchii. International Journal of Molecular Sciences, 22(7), 3524. https://doi.org/10.3390/ijms22073524

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