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Article

A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora

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Plant-Environment Interactions and Biodiversity Lab (PlantStress&Biodiversity), Forest Research Centre (CEF), Instituto Superior de Agronomia (ISA), Universidade de Lisboa, 2784-505 Oeiras and Tapada da Ajuda, 1349-017 Lisboa, Portugal
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Computational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
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GeoBioSciences, GeoTechnologies and GeoEngineering (GeoBioTec), Faculdade de Ciências e Tecnologia (FCT), Universidade NOVA de Lisboa (UNL), 2829-516 Monte de Caparica, Portugal
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Departamento de Biologia Vegetal, Universidade Federal Viçosa (UFV), Viçosa 36570-090, MG, Brazil
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Authors to whom correspondence should be addressed.
Academic Editor: Massimo Maffei
Int. J. Mol. Sci. 2021, 22(6), 3125; https://doi.org/10.3390/ijms22063125
Received: 1 February 2021 / Revised: 3 March 2021 / Accepted: 8 March 2021 / Published: 18 March 2021
(This article belongs to the Special Issue Tropical Plant Responses to Climate Change)
Understanding the effect of extreme temperatures and elevated air (CO2) is crucial for mitigating the impacts of the coffee industry. In this work, leaf transcriptomic changes were evaluated in the diploid C. canephora and its polyploid C. arabica, grown at 25 °C and at two supra-optimal temperatures (37 °C, 42 °C), under ambient (aCO2) or elevated air CO2 (eCO2). Both species expressed fewer genes as temperature rose, although a high number of differentially expressed genes (DEGs) were observed, especially at 42 °C. An enrichment analysis revealed that the two species reacted differently to the high temperatures but with an overall up-regulation of the photosynthetic machinery until 37 °C. Although eCO2 helped to release stress, 42 °C had a severe impact on both species. A total of 667 photosynthetic and biochemical related-DEGs were altered with high temperatures and eCO2, which may be used as key probe genes in future studies. This was mostly felt in C. arabica, where genes related to ribulose-bisphosphate carboxylase (RuBisCO) activity, chlorophyll a-b binding, and the reaction centres of photosystems I and II were down-regulated, especially under 42°C, regardless of CO2. Transcriptomic changes showed that both species were strongly affected by the highest temperature, although they can endure higher temperatures (37 °C) than previously assumed. View Full-Text
Keywords: climate changes; coffee; elevated air (CO2); functional analysis; high temperatures; leaf RNAseq; polyploidy; warming climate changes; coffee; elevated air (CO2); functional analysis; high temperatures; leaf RNAseq; polyploidy; warming
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MDPI and ACS Style

Marques, I.; Fernandes, I.; Paulo, O.S.; Lidon, F.C.; DaMatta, F.M.; Ramalho, J.C.; Ribeiro-Barros, A.I. A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora. Int. J. Mol. Sci. 2021, 22, 3125. https://doi.org/10.3390/ijms22063125

AMA Style

Marques I, Fernandes I, Paulo OS, Lidon FC, DaMatta FM, Ramalho JC, Ribeiro-Barros AI. A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora. International Journal of Molecular Sciences. 2021; 22(6):3125. https://doi.org/10.3390/ijms22063125

Chicago/Turabian Style

Marques, Isabel, Isabel Fernandes, Octávio S. Paulo, Fernando C. Lidon, Fábio M. DaMatta, José C. Ramalho, and Ana I. Ribeiro-Barros. 2021. "A Transcriptomic Approach to Understanding the Combined Impacts of Supra-Optimal Temperatures and CO2 Revealed Different Responses in the Polyploid Coffea arabica and Its Diploid Progenitor C. canephora" International Journal of Molecular Sciences 22, no. 6: 3125. https://doi.org/10.3390/ijms22063125

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