Next Article in Journal
Advances in Targeting HPV Infection as Potential Alternative Prophylactic Means
Previous Article in Journal
Exercise–Linked Irisin: Consequences on Mental and Cardiovascular Health in Type 2 Diabetes
Previous Article in Special Issue
Developmental Roles of FUSE Binding Protein 1 (Fubp1) in Tooth Morphogenesis
Open AccessArticle

Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta

1
Department of Diagnostic & Biomedical Sciences, School of Dentistry, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA
2
Center for Craniofacial Research, The University of Texas Health Science Center at Houston, Houston, TX 77054, USA
3
Center for Precision Health, School of Biomedical Informatics, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA
4
Human Genetics Center, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX 77030, USA
5
MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Bhagwati Gupta
Int. J. Mol. Sci. 2021, 22(4), 2202; https://doi.org/10.3390/ijms22042202
Received: 26 December 2020 / Revised: 14 February 2021 / Accepted: 15 February 2021 / Published: 23 February 2021
(This article belongs to the Special Issue Gene Networks That Control Cell Proliferation and Differentiation)
Amelogenesis imperfecta is a congenital form of enamel hypoplasia. Although a number of genetic mutations have been reported in humans, the regulatory network of these genes remains mostly unclear. To identify signatures of biological pathways in amelogenesis imperfecta, we conducted bioinformatic analyses on genes associated with the condition in humans. Through an extensive search of the main biomedical databases, we found 56 genes in which mutations and/or association/linkage were reported in individuals with amelogenesis imperfecta. These candidate genes were further grouped by function, pathway, protein–protein interaction, and tissue-specific expression patterns using various bioinformatic tools. The bioinformatic analyses highlighted a group of genes essential for extracellular matrix formation. Furthermore, advanced bioinformatic analyses for microRNAs (miRNAs), which are short non-coding RNAs that suppress target genes at the post-transcriptional level, predicted 37 candidates that may be involved in amelogenesis imperfecta. To validate the miRNA–gene regulation association, we analyzed the target gene expression of the top seven candidate miRNAs: miR-3195, miR-382-5p, miR-1306-5p, miR-4683, miR-6716-3p, miR-3914, and miR-3935. Among them, miR-1306-5p, miR-3195, and miR-3914 were confirmed to regulate ameloblast differentiation through the regulation of genes associated with amelogenesis imperfecta in AM-1 cells, a human ameloblastoma cell line. Taken together, our study suggests a potential role for miRNAs in amelogenesis imperfecta. View Full-Text
Keywords: microRNAs; tooth formation; amelogenesis imperfecta; enamel formation; tooth development microRNAs; tooth formation; amelogenesis imperfecta; enamel formation; tooth development
Show Figures

Figure 1

MDPI and ACS Style

Yoshioka, H.; Wang, Y.-Y.; Suzuki, A.; Shayegh, M.; Gajera, M.V.; Zhao, Z.; Iwata, J. Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta. Int. J. Mol. Sci. 2021, 22, 2202. https://doi.org/10.3390/ijms22042202

AMA Style

Yoshioka H, Wang Y-Y, Suzuki A, Shayegh M, Gajera MV, Zhao Z, Iwata J. Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta. International Journal of Molecular Sciences. 2021; 22(4):2202. https://doi.org/10.3390/ijms22042202

Chicago/Turabian Style

Yoshioka, Hiroki; Wang, Yin-Ying; Suzuki, Akiko; Shayegh, Meysam; Gajera, Mona V.; Zhao, Zhongming; Iwata, Junichi. 2021. "Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta" Int. J. Mol. Sci. 22, no. 4: 2202. https://doi.org/10.3390/ijms22042202

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop