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Open AccessArticle

Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation

1
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Science, Kunming 650201, China
2
Royal Botanic Gardens, Kew, Wellcome Trust Millennium Building, Wakehurst Place, West Sussex, Ardingly RH17 6TN, UK
*
Authors to whom correspondence should be addressed.
Academic Editor: Elsa Arcalis
Int. J. Mol. Sci. 2021, 22(4), 2174; https://doi.org/10.3390/ijms22042174
Received: 20 January 2021 / Revised: 12 February 2021 / Accepted: 15 February 2021 / Published: 22 February 2021
(This article belongs to the Special Issue Morphology and Physiology of Seeds and Other Plant Storage Tissues)
Plant species conservation through cryopreservation using plant vitrification solutions (PVS) is based in empiricism and the mechanisms that confer cell integrity are not well understood. Using ESI-MS/MS analysis and quantification, we generated 12 comparative lipidomics datasets for membranes of embryogenic cells (ECs) of Magnolia officinalis during cryogenic treatments. Each step of the complex PVS-based cryoprotocol had a profoundly different impact on membrane lipid composition. Loading treatment (osmoprotection) remodeled the cell membrane by lipid turnover, between increased phosphatidic acid (PA) and phosphatidylglycerol (PG) and decreased phosphatidylcholine (PC) and phosphatidylethanolamine (PE). The PA increase likely serves as an intermediate for adjustments in lipid metabolism to desiccation stress. Following PVS treatment, lipid levels increased, including PC and PE, and this effectively counteracted the potential for massive loss of lipid species when cryopreservation was implemented in the absence of cryoprotection. The present detailed cryobiotechnology findings suggest that the remodeling of membrane lipids and attenuation of lipid degradation are critical for the successful use of PVS. As lipid metabolism and composition varies with species, these new insights provide a framework for technology development for the preservation of other species at increasing risk of extinction. View Full-Text
Keywords: cryopreservation; cryoprotectant agents; ex situ conservation; lipid; Magnoliaceae; plant vitrification solutions cryopreservation; cryoprotectant agents; ex situ conservation; lipid; Magnoliaceae; plant vitrification solutions
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MDPI and ACS Style

Lin, L.; Ma, J.; Ai, Q.; Pritchard, H.W.; Li, W.; Chen, H. Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation. Int. J. Mol. Sci. 2021, 22, 2174. https://doi.org/10.3390/ijms22042174

AMA Style

Lin L, Ma J, Ai Q, Pritchard HW, Li W, Chen H. Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation. International Journal of Molecular Sciences. 2021; 22(4):2174. https://doi.org/10.3390/ijms22042174

Chicago/Turabian Style

Lin, Liang; Ma, Junchao; Ai, Qin; Pritchard, Hugh W.; Li, Weiqi; Chen, Hongying. 2021. "Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation" Int. J. Mol. Sci. 22, no. 4: 2174. https://doi.org/10.3390/ijms22042174

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