Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior
Abstract
:1. Introduction
2. Results
2.1. Urothelium-Specific Deletion of Connexin43
2.2. Evaluation of Mechanosensitive Urothelial ATP Release in uCx43KO Mice
2.3. Characterization of the Voiding Behavior of uCx43KO Mice
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Immunohistochemistry of Mouse Bladder
4.3. Tissue Harvesting
4.4. Immunoblotting
4.5. Bladder Distension and Quantification of Luminal ATP Release
4.6. Micturition Analysis
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Kono, J.; Ueda, M.; Sengiku, A.; Suadicani, S.O.; Ogawa, O.; Negoro, H. Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior. Int. J. Mol. Sci. 2021, 22, 1594. https://doi.org/10.3390/ijms22041594
Kono J, Ueda M, Sengiku A, Suadicani SO, Ogawa O, Negoro H. Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior. International Journal of Molecular Sciences. 2021; 22(4):1594. https://doi.org/10.3390/ijms22041594
Chicago/Turabian StyleKono, Jin, Masakatsu Ueda, Atsushi Sengiku, Sylvia O. Suadicani, Osamu Ogawa, and Hiromitsu Negoro. 2021. "Urothelium-Specific Deletion of Connexin43 in the Mouse Urinary Bladder Alters Distension-Induced ATP Release and Voiding Behavior" International Journal of Molecular Sciences 22, no. 4: 1594. https://doi.org/10.3390/ijms22041594