Impact of Cigarette Smoking on the Expression of Oxidative Stress-Related Genes in Cumulus Cells Retrieved from Healthy Women Undergoing IVF
Abstract
:1. Introduction
2. Results
2.1. Patients’ Characteristics
2.2. Gene Expression
2.3. Functional and Network Analysis
2.4. IL-6 Promoter Methylation through Pyrosequencing
3. Discussion
4. Materials and Methods
4.1. Ethical Approval
4.2. Source of Cumulus Cells and Patients’ Selection Criteria
4.3. Ovarian Stimulation Protocols
4.4. Cumulus Cells Isolation
4.5. Quantitative Real-Time PCR
4.6. IPA-Inferred Biological Networks and Upstream Regulators Analysis
4.7. Bisulfite Conversion and DNA Methylation by Pyrosequencing
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Limitations
References
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Non Smokers (n = 5) | Smokers (n = 5) | p-Value | |
---|---|---|---|
Mean n° of cigarettes smoked/daily | - | 8.2 | |
Age | 30.8 ± 0.8 | 33.2 ± 2.6 | 0.08 |
AMH | 8.6 ± 2.5 | 9.2 ± 8.3 | 0.9 |
BMI | 25.3 ± 4.5 | 24.6 ± 5.4 | 0.8 |
Previous IVF attempts | 1.4 ± 0.5 | 1.4 ± 0.5 | 1 |
Days of stimulation | 9.4 ± 2.1 | 9.8 ± 0.8 | 0.7 |
Total dosage of gonadotropin | 1260.0 ± 697.6 | 1695.0 ± 1336.8 | 0.5 |
Oocytes retrieved at ovum pick-up | 11.6 ± 2.1 | 12.2 ± 8.9 | 0.9 |
N° of MII oocytes | 8.4 ± 1.9 | 8.8 ± 7.4 | 0.9 |
ID | Expr Log Ratio | Entrez Gene Name | Location | Type(s) |
---|---|---|---|---|
APEX1 | −0.534 | apurinic/apyrimidinic endodeoxyribonuclease 1 | Nucleus | enzyme |
BCL2 | −0.528 | BCL2 apoptosis regulator | Cytoplasm | transporter |
CAT | −0.422 | catalase | Cytoplasm | enzyme |
CYBA | −0.414 | cytochrome b-245 alpha chain | Cytoplasm | enzyme |
EGFR | −0.480 | epidermal growth factor receptor | Plasma Membrane | kinase |
F3 | −0.540 | coagulation factor III, tissue factor | Plasma Membrane | transmembrane receptor |
FOXO1 | −0.435 | forkhead box O1 | Nucleus | transcription regulator |
GCLC | −0.461 | glutamate-cysteine ligase catalytic subunit | Cytoplasm | enzyme |
GPX3 | −0.416 | glutathione peroxidase 3 | Extracellular Space | enzyme |
HIF1A | −0.539 | hypoxia inducible factor 1 subunit alpha | Nucleus | transcription regulator |
HMOX1 | −0.408 | heme oxygenase 1 | Cytoplasm | enzyme |
IL1B | −0.335 | interleukin 1 beta | Extracellular Space | cytokine |
IL6 | 2.432 | interleukin 6 | Extracellular Space | cytokine |
JUN | −0.548 | Jun proto-oncogene, AP-1 transcription factor subunit | Nucleus | transcription regulator |
MAP3K5 | −0.533 | mitogen-activated protein kinase kinase kinase 5 | Cytoplasm | kinase |
MAPK1 | −0.536 | mitogen-activated protein kinase 1 | Cytoplasm | kinase |
MAPK8 | −0.469 | mitogen-activated protein kinase 8 | Cytoplasm | kinase |
MSRA | −0.452 | methionine sulfoxide reductase A | Cytoplasm | enzyme |
NFE2L2 | −0.445 | nuclear factor, erythroid 2 like 2 | Nucleus | transcription regulator |
NFKB1 | −0.386 | nuclear factor kappa B subunit 1 | Nucleus | transcription regulator |
NQO1 | −0.495 | NAD(P)H quinone dehydrogenase 1 | Cytoplasm | enzyme |
SERPINE1 | −0.402 | serpin family E member 1 | Extracellular Space | other |
SIRT1 | −0.544 | sirtuin 1 | Nucleus | transcription regulator |
SOD2 | 1.520 | superoxide dismutase 2 | Cytoplasm | enzyme |
STAT1 | −0.513 | signal transducer and activator of transcription 1 | Nucleus | transcription regulator |
TP53 | −0.364 | tumor protein p53 | Nucleus | transcription regulator |
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Konstantinidou, F.; Budani, M.C.; Sarra, A.; Stuppia, L.; Tiboni, G.M.; Gatta, V. Impact of Cigarette Smoking on the Expression of Oxidative Stress-Related Genes in Cumulus Cells Retrieved from Healthy Women Undergoing IVF. Int. J. Mol. Sci. 2021, 22, 13147. https://doi.org/10.3390/ijms222313147
Konstantinidou F, Budani MC, Sarra A, Stuppia L, Tiboni GM, Gatta V. Impact of Cigarette Smoking on the Expression of Oxidative Stress-Related Genes in Cumulus Cells Retrieved from Healthy Women Undergoing IVF. International Journal of Molecular Sciences. 2021; 22(23):13147. https://doi.org/10.3390/ijms222313147
Chicago/Turabian StyleKonstantinidou, Fani, Maria Cristina Budani, Annalina Sarra, Liborio Stuppia, Gian Mario Tiboni, and Valentina Gatta. 2021. "Impact of Cigarette Smoking on the Expression of Oxidative Stress-Related Genes in Cumulus Cells Retrieved from Healthy Women Undergoing IVF" International Journal of Molecular Sciences 22, no. 23: 13147. https://doi.org/10.3390/ijms222313147