Evaluation of the Physico-Chemical Properties of Liposomes Assembled from Bioconjugates of Anisic Acid with Phosphatidylcholine
Abstract
:1. Introduction
2. Results and Discussion
2.1. Differential Scanning Calorimetry (DSC)
2.2. Spectroscopy Methods
2.2.1. Steady-State Fluorescence Spectroscopy
2.2.2. Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR)
3. Summary
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Liposome Preparation
4.3. Differential Scanning Calorimetry (DSC)
4.4. Spectroscopy Methods
4.4.1. Steady-State Fluorescence Spectroscopy
4.4.2. Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR)
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ATR Spectra, Wavenumbers (cm−1) | |||
---|---|---|---|
Assigned * | DPPC | 1-ANISA-2-PA-PC/DPPC | 1-PA-2-ANISA-PC/DPPC |
25 °C | |||
δ(CH2) | 1467.66 | 1467.53 | 1467.37 |
γ(CH2) | 720.44 | 720.88 | 721.06 |
νs(N-C) | 925.39 | 925.39 | 926.07 |
νas(N-C)ip | 970.31 | 970.93 | 969.85 |
νs(COP) | 1064.92 | 1066.32 | 1064.65 |
νs(PO2−) | 1087.80 | 1088.40 | 1088.94 |
νas(PO2−) | 1221.61 | 1221.47 | 1221.82 |
ν(C=O) | 1733.94 | 1733.52 | 1733.74 |
νs(CH2) | 2849.71 | 2849.69 | 2849.62 |
νs(CH3) | 2872.91 | 2872.75 | 2872.83 |
νas(CH2) | 2917.26 | 2917.13 | 2917.12 |
νas(CH3) | 2955.57 | 2955.53 | 2955.53 |
38 °C | |||
δ(CH2) | 1467.73 | 1467.63 | 1467.69 |
γr(CH2) | 720.44 | 721.08 | 721.06 |
νs(N-C) | 925.39 | 925.95 | 926.07 |
νas(N-C)ip | 969.61 | 971.90 | 969.85 |
νs(COP) | 1066.61 | 1068.58 | 1068.44 |
νs(PO2−) | 1087.69 | 1087.49 | 1087.24 |
νas(PO2−) | 1221.86 | 1225.89 | 1221.27 |
ν(C=O) | 1736.55 | 1735.46 | 1733.50 |
νs(CH2) | 2849.87 | 2851.71 | 2850.40 |
νs(CH3) | 2872.73 | 2872.63 | 2872.68 |
νas(CH2) | 2917.71 | 2920.99 | 2918.63 |
νas(CH3) | 2956.57 | 2956.53 | 2956.31 |
50 °C | |||
δ(CH2) | 1465.32 | 1464.78 | 1464.88 |
γr(CH2) | ND | ND | ND |
νs(N-C) | ND | ND | ND |
νas N-C)ip | 967.77 | 971.81 | 969.85 |
νs(COP) | 1068.22 | 1069.32 | 1068.22 |
νs(PO2−) | 1087.02 | 1086.95 | 1086.22 |
νas(PO2−) | 1220.47 | 1215.09 | 1215.48 |
ν(C=O) | 1731.56 | ND | ND |
νs(CH2) | 2852.74 | 2852.82 | 2852.70 |
νs(CH3) | ND | ND | ND |
νas(CH2) | 2922.06 | 2922.74 | 2922.53 |
νas(CH3) | 2956.56 | 2956.32 | 2956.45 |
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Pruchnik, H.; Gliszczyńska, A.; Włoch, A. Evaluation of the Physico-Chemical Properties of Liposomes Assembled from Bioconjugates of Anisic Acid with Phosphatidylcholine. Int. J. Mol. Sci. 2021, 22, 13146. https://doi.org/10.3390/ijms222313146
Pruchnik H, Gliszczyńska A, Włoch A. Evaluation of the Physico-Chemical Properties of Liposomes Assembled from Bioconjugates of Anisic Acid with Phosphatidylcholine. International Journal of Molecular Sciences. 2021; 22(23):13146. https://doi.org/10.3390/ijms222313146
Chicago/Turabian StylePruchnik, Hanna, Anna Gliszczyńska, and Aleksandra Włoch. 2021. "Evaluation of the Physico-Chemical Properties of Liposomes Assembled from Bioconjugates of Anisic Acid with Phosphatidylcholine" International Journal of Molecular Sciences 22, no. 23: 13146. https://doi.org/10.3390/ijms222313146