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Article

The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L

1
Department of Anatomy and Comparative Pathological Anatomy, Faculty of Veterinary, Campus of Espinardo, University of Murcia, 30100 Murcia, Spain
2
Department of Cell Biology and Histology, Faculty of Biology, Campus of Espinardo, University of Murcia, 30100 Murcia, Spain
3
Centro Oceanográfico de Murcia, Instituto Español de Oceanografía (IEO-CSIC), Puerto de Mazarrón, 30860 Murcia, Spain
4
Department of Cell Biology, Physiology and Immunology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Vincenzo Sorrentino
Int. J. Mol. Sci. 2021, 22(23), 13118; https://doi.org/10.3390/ijms222313118
Received: 5 November 2021 / Revised: 29 November 2021 / Accepted: 2 December 2021 / Published: 4 December 2021
(This article belongs to the Special Issue Endocrine Control of Fish Metabolism)
Endocrine-disrupting chemicals include natural and synthetic estrogens, such as 17α-ethynilestradiol (EE2), which can affect reproduction, growth and immunity. Estrogen signalling is mediated by nuclear or membrane estrogen receptors, such as the new G-protein-coupled estrogen receptor 1 (GPER1). The present work studies the effect of EE2 and G1 (an agonist of GPER1) on body and muscle parameters and growth-related genes of 54 two-year-old seabreams. The fish were fed a diet containing EE2 (EE2 group) and G1 (G1 group) for 45 days and then a diet without EE2 or G1 for 122 days. An untreated control group was also studied. At 45 days, the shortest body length was observed in the G1 group, while 79 and 122 days after the cessation of treatments, the shortest body growth was observed in the EE2 group. Hypertrophy of white fibers was higher in the EE2 and G1 groups than it was in the control group, whereas the opposite was the case with respect to hyperplasia. Textural hardness showed a negative correlation with the size of white fibers. At the end of the experiment, all fish analyzed in the EE2 group showed a predominance of the gonadal ovarian area. In addition, the highest expression of the mafbx gene (upregulated in catabolic signals) and mstn2 (myogenesis negative regulator) was found in EE2-exposed fish. View Full-Text
Keywords: EE2; GPER1; body growth; muscle cellularity; muscle growth regulator genes EE2; GPER1; body growth; muscle cellularity; muscle growth regulator genes
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MDPI and ACS Style

Ayala, M.D.; Gómez, V.; Cabas, I.; García Hernández, M.P.; Chaves-Pozo, E.; Arizcun, M.; Garcia de la Serrana, D.; Gil, F.; García-Ayala, A. The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L. Int. J. Mol. Sci. 2021, 22, 13118. https://doi.org/10.3390/ijms222313118

AMA Style

Ayala MD, Gómez V, Cabas I, García Hernández MP, Chaves-Pozo E, Arizcun M, Garcia de la Serrana D, Gil F, García-Ayala A. The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L. International Journal of Molecular Sciences. 2021; 22(23):13118. https://doi.org/10.3390/ijms222313118

Chicago/Turabian Style

Ayala, Maria D., Victoria Gómez, Isabel Cabas, María P. García Hernández, Elena Chaves-Pozo, Marta Arizcun, Daniel Garcia de la Serrana, Francisco Gil, and Alfonsa García-Ayala. 2021. "The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L" International Journal of Molecular Sciences 22, no. 23: 13118. https://doi.org/10.3390/ijms222313118

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