Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production
Abstract
:1. Introduction
2. Results
2.1. Secretome from Statically Loaded Myoblasts Enhances Tenocyte Migration
2.2. Mechanically Loaded Myoblasts Have No Effects on Tenocyte Proliferation and Apoptosis
2.3. Secretome from Mechanically Loaded Myoblasts Alters Collagen Expression of Tenocytes
2.4. Statically Loaded Myoblasts Change the Expression of Tenocyte Markers
3. Discussion
4. Materials and Methods
4.1. Isolation and Culture of Primary Cells
4.2. Mechanical Strain
4.3. Indirect Co-Culture
4.4. Migration Assays
4.5. Cell proliferative Assay
4.6. Lactate Dehydrogenase Activity Assay
4.7. RNA Extraction and qRT-PCR
4.8. Western Blot
4.9. ELISA
4.10. Immunocytochemistry
4.11. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Gene Symbol | Gene Name | Assay ID |
---|---|---|
Mcam | melanoma cell adhesion molecule, CD 146 | Rn00576900_m1 |
Vim | Vimentin | Rn00579738 |
Tnc | Tenascin-C | Rn01454948 |
Acta2 | actin alpha 2, smooth muscle, α-SMA | Rn01759928 |
S100a4 | S100 calcium-binding protein A4 | Rn01451938 |
Scx | Scleraxis bHLH transcription factor | Rn01504576 |
Col1a1 | Collagen type I alpha 1 chain | Rn01463848_m1 |
Col3a1 | collagen type III alpha 1 chain | Rn01437681_m1 |
Rpl13a (reference gene) | Ribosomal Protein L13a | Rn00821946_g1 |
Antibody | Company | Code | Dilution | Species | Molecular Weight (kDa) |
---|---|---|---|---|---|
PCNA | Cell Signaling | 2586 | 1:1000 | Mouse | 36 |
c-PARP | Cell Signaling | 9541 | 1:1000 | Rabbit | 89 |
β-actin | Cell Signaling | 4967 | 1:2000 | Rabbit | 45 |
Anti-rabbit IgG, HRP-linked antibody | Cell Signaling | 7074 | 1:2000 | Goat |
Antibody | Company | Code | Dilution | Species |
---|---|---|---|---|
Collagen I | Abcam | 260043 | 1:400 | Rabbit |
α-SMA | Thermo Fisher | 53976082 | 1:400 | Rabbit |
Fluorescein-conjugated secondary antibody Goat-anti rabbit | Thermo Fisher | A32740 | 1:400 | Rabbit |
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Zhou, X.; Li, J.; Giannopoulos, A.; Kingham, P.J.; Backman, L.J. Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production. Int. J. Mol. Sci. 2021, 22, 13089. https://doi.org/10.3390/ijms222313089
Zhou X, Li J, Giannopoulos A, Kingham PJ, Backman LJ. Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production. International Journal of Molecular Sciences. 2021; 22(23):13089. https://doi.org/10.3390/ijms222313089
Chicago/Turabian StyleZhou, Xin, Junhong Li, Antonios Giannopoulos, Paul J. Kingham, and Ludvig J. Backman. 2021. "Secretome from In Vitro Mechanically Loaded Myoblasts Induces Tenocyte Migration, Transition to a Fibroblastic Phenotype and Suppression of Collagen Production" International Journal of Molecular Sciences 22, no. 23: 13089. https://doi.org/10.3390/ijms222313089