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Article

Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model

by 1,2,3, 1,2,3, 1,2,3 and 1,2,3,*
1
Centre de Recherche en Organogénèse Expérimentale de l’Université Laval/LOEX, Québec, QC G1J 1Z4, Canada
2
Axe Médecine Régénératrice, Centre de Recherche du CHU de Québec-Université Laval, Québec, QC G1J 1Z4, Canada
3
Faculté de Pharmacie de l’Université Laval, Québec, QC G1V 0A6, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: Daniela Marasco
Int. J. Mol. Sci. 2021, 22(23), 13091; https://doi.org/10.3390/ijms222313091
Received: 4 October 2021 / Revised: 24 November 2021 / Accepted: 29 November 2021 / Published: 3 December 2021
Healthy skin moLEdels produced by tissue-engineering often present a suboptimal skin barrier function as compared with normal human skin. Moreover, skin substitutes reconstructed according to the self-assembly method were found to be deficient in polyunsaturated fatty acids (PUFAs). Therefore, in this study, we investigated the effects of a supplementation of the culture media with docosahexaenoic acid (DHA) on the barrier function of skin substitutes. To this end, 10 μM DHA-supplemented skin substitutes were produced (n = 3), analyzed, and compared with controls (substitutes without supplementation). A Franz cell diffusion system, followed by ultra-performance liquid chromatography, was used to perform a skin permeability to testosterone assay. We then used gas chromatography to quantify the PUFAs found in the epidermal phospholipid fraction of the skin substitutes, which showed successful DHA incorporation. The permeability to testosterone was decreased following DHA supplementation and the lipid profile was improved. Differences in the expression of the tight junction (TJ) proteins claudin-1, claudin-4, occludin, and TJ protein-1 were observed, principally a significant increase in claudin-1 expression, which was furthermore confirmed by Western blot analyses. In conclusion, these results confirm that the DHA supplementation of cell culture media modulates different aspects of skin barrier function in vitro and reflects the importance of n-3 PUFAs regarding the lipid metabolism in keratinocytes. View Full-Text
Keywords: skin substitutes; skin barrier function; tight junctions; polyunsaturated fatty acids; docosahexaenoic acid; tissue engineering; lipidomics skin substitutes; skin barrier function; tight junctions; polyunsaturated fatty acids; docosahexaenoic acid; tissue engineering; lipidomics
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MDPI and ACS Style

Tremblay, A.; Simard, M.; Morin, S.; Pouliot, R. Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model. Int. J. Mol. Sci. 2021, 22, 13091. https://doi.org/10.3390/ijms222313091

AMA Style

Tremblay A, Simard M, Morin S, Pouliot R. Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model. International Journal of Molecular Sciences. 2021; 22(23):13091. https://doi.org/10.3390/ijms222313091

Chicago/Turabian Style

Tremblay, Andréa, Mélissa Simard, Sophie Morin, and Roxane Pouliot. 2021. "Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model" International Journal of Molecular Sciences 22, no. 23: 13091. https://doi.org/10.3390/ijms222313091

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