Olfactory Receptor OR7A17 Expression Correlates with All-Trans Retinoic Acid (ATRA)-Induced Suppression of Proliferation in Human Keratinocyte Cells
Abstract
:1. Introduction
2. Results
2.1. ATRA Downregulated the Expression of OR7A17 in Human Keratinocytes
2.2. ATRA Suppresses the Proliferation of HaCaT Cells
2.3. OR7A17 Expression Is Regulated via RAR Signaling
2.4. Overexpression of OR7A17 Interferes with the Suppressive Effect of ATRA
2.5. OR7A17 Overexpression Induces Proliferation of HaCaT Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. ATRA Treatment
4.3. Fluo-4 Ca2+ Influx Assay
4.4. Luciferase Reporter Assay and β-Galactosidase Activity Assay
4.5. Western Blotting Analysis
4.6. Antibodies
4.7. Reverse Transcription qPCR (RT-qPCR)
4.8. EdU Incorporation Assay
4.9. CellTiter Glo® 2.0 Assay for Cell Proliferation Analysis
4.10. Lentiviral Transduction of HaCaT Cells
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, H.; Park, S.-H.; Oh, S.W.; Kwon, K.; Park, S.J.; Yu, E.; Yang, S.; Park, J.Y.; Choi, S.; Yang, S.; et al. Olfactory Receptor OR7A17 Expression Correlates with All-Trans Retinoic Acid (ATRA)-Induced Suppression of Proliferation in Human Keratinocyte Cells. Int. J. Mol. Sci. 2021, 22, 12304. https://doi.org/10.3390/ijms222212304
Kim H, Park S-H, Oh SW, Kwon K, Park SJ, Yu E, Yang S, Park JY, Choi S, Yang S, et al. Olfactory Receptor OR7A17 Expression Correlates with All-Trans Retinoic Acid (ATRA)-Induced Suppression of Proliferation in Human Keratinocyte Cells. International Journal of Molecular Sciences. 2021; 22(22):12304. https://doi.org/10.3390/ijms222212304
Chicago/Turabian StyleKim, Hyeyoun, See-Hyoung Park, Sae Woong Oh, Kitae Kwon, Se Jung Park, Eunbi Yu, Seyoung Yang, Jung Yoen Park, Seoyoung Choi, Seoyoun Yang, and et al. 2021. "Olfactory Receptor OR7A17 Expression Correlates with All-Trans Retinoic Acid (ATRA)-Induced Suppression of Proliferation in Human Keratinocyte Cells" International Journal of Molecular Sciences 22, no. 22: 12304. https://doi.org/10.3390/ijms222212304