Mixed Transcriptome Analysis Revealed the Possible Interaction Mechanisms between Zizania latifolia and Ustilago esculenta Inducing Jiaobai Stem-Gall Formation
Abstract
:1. Introduction
2. Results
2.1. Distribution of U. esculenta in the Initial Trigger of Stem-Gall Formation of Z. latifolia
2.2. General Features of the U. esculenta and Z. latifolia Transcriptomes
2.3. Analysis of Pathogenicity Genes in U. esculenta and Plant Resistance Genes in Z. latifolia
2.4. Hormonal Analysis during Stem-Gall Formation in Z. latifolia
2.4.1. Hormone Content Analysis
2.4.2. DEGs Involved in Hormone Metabolism and Signal Transduction
2.5. Auxin Regulation of Stem-Gall Formation in Z. latifolia
2.5.1. Genes Encoding Enzymes Involved in IAA Biosynthesis in U. esculenta and Z. latifolia
2.5.2. Structural and Functional Characteristic Analysis of the YUCCA Gene Family in Z. latifolia
2.5.3. Gene Expression of Enzymes Related to IAA Biosynthesis at the Early Stage of Stem-Gall Formation by qRT-PCR
2.6. Expression Pattern of Cys2–His2 (C2H2) Zinc Finger Proteins of U. esculenta at the Early Stage of Stem-Gall Formation
3. Discussion
4. Materials and Methods
4.1. Plant Material and Sample Collection
4.2. Distribution of Hyphae in Z. latifolia
4.3. RNA Isolation, mRNA-Seq Library Preparation and Sequencing
4.4. Preprocessing of mRNA Sequence Data
4.5. Functional Annotation of DEGs
4.6. ESI-HPLC-MS/MS Assay of Endogenous Hormones
4.7. Genome-Wide Identification of the IAA Synthesis Genes and C2H2 Zinc Finger Proteins in U. esculenta and Z. latifolia
4.8. Confirmation of DEGs by qRT-PCR
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Statistics | U. esculenta | Z. latifolia |
---|---|---|
Total clean reads (Gb) | 15.25 | 15.25 |
Average mapping ratio of genome sequence (%) | 4.76 | 84.03 |
Average mapping ratio of gene set (%) | 3.56 | 66.82 |
Total gene number | 6330 | 40,856 |
Known gene number | 6319 | 37,622 |
Novel gene number | 11 | 3234 |
Total novel transcript | 1345 | 36,704 |
Coding transcript | 1013 | 28,273 |
Noncoding transcript | 332 | 8431 |
Novel isoform | 1002 | 24,993 |
Novel transcript | 11 | 3280 |
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Zhang, Z.-P.; Song, S.-X.; Liu, Y.-C.; Zhu, X.-R.; Jiang, Y.-F.; Shi, L.-T.; Jiang, J.-Z.; Miao, M.-M. Mixed Transcriptome Analysis Revealed the Possible Interaction Mechanisms between Zizania latifolia and Ustilago esculenta Inducing Jiaobai Stem-Gall Formation. Int. J. Mol. Sci. 2021, 22, 12258. https://doi.org/10.3390/ijms222212258
Zhang Z-P, Song S-X, Liu Y-C, Zhu X-R, Jiang Y-F, Shi L-T, Jiang J-Z, Miao M-M. Mixed Transcriptome Analysis Revealed the Possible Interaction Mechanisms between Zizania latifolia and Ustilago esculenta Inducing Jiaobai Stem-Gall Formation. International Journal of Molecular Sciences. 2021; 22(22):12258. https://doi.org/10.3390/ijms222212258
Chicago/Turabian StyleZhang, Zhi-Ping, Si-Xiao Song, Yan-Cheng Liu, Xin-Rui Zhu, Yi-Feng Jiang, Ling-Tong Shi, Jie-Zeng Jiang, and Min-Min Miao. 2021. "Mixed Transcriptome Analysis Revealed the Possible Interaction Mechanisms between Zizania latifolia and Ustilago esculenta Inducing Jiaobai Stem-Gall Formation" International Journal of Molecular Sciences 22, no. 22: 12258. https://doi.org/10.3390/ijms222212258