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Article

Jasmonic Acid-Dependent MYC Transcription Factors Bind to a Tandem G-Box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses

1
Centro de Biotecnología y Genómica de Plantas, Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA), Universidad Politécnica de Madrid (UPM), 28223 Pozuelo de Alarcón, Spain
2
Department of Forest Genetics and Plant Physiology, Umeå Plant Sciences Centre (UPSC), Swedish University of Agricultural Science, 90183 Umeå, Sweden
3
Institut für Biologie, Bereich Pflanzenwissenschaften, Karl-Franzens Universität Graz, 8010 Graz, Austria
4
Departamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid (UPM), 28040 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Ryszard Lobinski
Int. J. Mol. Sci. 2021, 22(18), 9768; https://doi.org/10.3390/ijms22189768
Received: 2 July 2021 / Revised: 6 September 2021 / Accepted: 7 September 2021 / Published: 9 September 2021
The indole-3-pyruvic acid pathway is the main route for auxin biosynthesis in higher plants. Tryptophan aminotransferases (TAA1/TAR) and members of the YUCCA family of flavin-containing monooxygenases catalyze the conversion of l-tryptophan via indole-3-pyruvic acid to indole-3-acetic acid (IAA). It has been described that jasmonic acid (JA) locally produced in response to mechanical wounding triggers the de novo formation of IAA through the induction of two YUCCA genes, YUC8 and YUC9. Here, we report the direct involvement of a small number of basic helix-loop-helix transcription factors of the MYC family in this process. We show that the JA-mediated regulation of the expression of the YUC8 and YUC9 genes depends on the abundance of MYC2, MYC3, and MYC4. In support of this observation, seedlings of myc knockout mutants displayed a strongly reduced response to JA-mediated IAA formation. Furthermore, transactivation assays provided experimental evidence for the binding of MYC transcription factors to a particular tandem G-box motif abundant in the promoter regions of YUC8 and YUC9, but not in the promoters of the other YUCCA isogenes. Moreover, we demonstrate that plants that constitutively overexpress YUC8 and YUC9 show less damage after spider mite infestation, thereby underlining the role of auxin in plant responses to biotic stress signals. View Full-Text
Keywords: Arabidopsis thaliana; indole-3-acetic acid; jasmonic acid; plant hormone crosstalk; transcriptional regulation; wound response; biotic stress; growth-defense trade-off Arabidopsis thaliana; indole-3-acetic acid; jasmonic acid; plant hormone crosstalk; transcriptional regulation; wound response; biotic stress; growth-defense trade-off
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MDPI and ACS Style

Pérez-Alonso, M.-M.; Sánchez-Parra, B.; Ortiz-García, P.; Santamaría, M.E.; Díaz, I.; Pollmann, S. Jasmonic Acid-Dependent MYC Transcription Factors Bind to a Tandem G-Box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses. Int. J. Mol. Sci. 2021, 22, 9768. https://doi.org/10.3390/ijms22189768

AMA Style

Pérez-Alonso M-M, Sánchez-Parra B, Ortiz-García P, Santamaría ME, Díaz I, Pollmann S. Jasmonic Acid-Dependent MYC Transcription Factors Bind to a Tandem G-Box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses. International Journal of Molecular Sciences. 2021; 22(18):9768. https://doi.org/10.3390/ijms22189768

Chicago/Turabian Style

Pérez-Alonso, Marta-Marina, Beatriz Sánchez-Parra, Paloma Ortiz-García, Maria Estrella Santamaría, Isabel Díaz, and Stephan Pollmann. 2021. "Jasmonic Acid-Dependent MYC Transcription Factors Bind to a Tandem G-Box Motif in the YUCCA8 and YUCCA9 Promoters to Regulate Biotic Stress Responses" International Journal of Molecular Sciences 22, no. 18: 9768. https://doi.org/10.3390/ijms22189768

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