Activated Carbon Fiber Cloth/Biomimetic Apatite: A Dual Drug Delivery System
Abstract
:1. Introduction
2. Results and Discussion
2.1. Apatite Material Characterization
2.2. Characterization of Activated Carbon Fiber Cloth (ACC) Substrates
2.3. Characterization of ACC/CDA Porosity
2.4. Tetracycline and Aspirin Adsorption Experiments
2.4.1. Adsorption Kinetics and Isotherms on Biomimetic Apatite Powder
2.4.2. Adsorption Kinetics and Isotherms on ACC Material
2.4.3. Adsorption Kinetics on ACC/CDA Material
2.4.4. Double Adsorption on ACC/CDA Material
2.4.5. Desorption/Release Study
2.5. In Vitro Biocompatibility Assays on Human Osteoblasts
2.6. In Vivo Effect of ACC/CDA-AA Material on Cortical Bone Regeneration
3. Materials and Methods
3.1. Materials
3.1.1. Apatites
3.1.2. Activated Carbon Fiber Cloth (ACC) Substrates
3.1.3. Drugs
3.2. Drug Adsorption and Release Experiments
3.2.1. Drug Adsorption on Biomimetic Apatite Powder
3.2.2. Drug Adsorption on ACC and ACC/CDA Composite Materials
3.2.3. Double Drug Adsorption on ACC/CDA Composite Material
3.2.4. Drug Release
3.3. In Vitro Procedures
3.4. In Vivo Procedures
3.4.1. Surgical Procedure
3.4.2. AA Adsorption during Surgical Procedure
3.5. Micro-Computed Tomography Analysis
3.6. Statistical Analyses
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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CO3 %wt. | Morphology (SEM) | Structure (XRD) | Lattice Parameters | |||
---|---|---|---|---|---|---|
a (Å) | b (Å) | |||||
“Reference” nanocrystalline apatite | 1.46 ± 0.02 | 0 | Plate-like | Hexagonal, (P63/m) | 9.437 ± 0.004 | 6.871 ± 0.002 |
Carbonated CDA | 1.4 ± 0.1 | 2–6 | Plate-like | Hexagonal, (P63/m) | 9.366 | 6.820 |
Precursor | Sizing | Architecture | Fiber Diameter (µm) | SBET (m2/g) | VTOTAL (cm3/g) | Acidic Functional Groups (mmol/g) | pHpzc | |
---|---|---|---|---|---|---|---|---|
ACC 1 | Phenolic resin | – | Woven | 10 | 1693 | 0.68 | 0.48 | 9.4 |
ACC 2 | Viscose | ZnAl2O4 | Knitted | 8–12 | 898 | 0.51 | 2.93 | 7.8 |
ACC 3 | Viscose | ZnAl2O4 | Knitted | 12–15 | 1400 | 1.27 | 1.31 | 5.8 |
SBET (m2/g) | Vmicro 1 N2, DFT (cm3/g) | Vmeso 1 N2, DFT (cm3/g) | Vmicro 2 N2, DR (cm3/g) | |
---|---|---|---|---|
ACC | 898 | 0.41 | 0.07 | 0.41 |
ACC/CDA | 980 | 0.45 | 0.10 | 0.46 |
Chemical Formula | M (g/mol) | Volume (Å3) | pKa (25 °C) | Log P | Log D (pH = 7.4) | Polar Surface Area (Å2) | |
---|---|---|---|---|---|---|---|
Tetracycline (TC) | 444.43 | 449 | 3.3 | −1.47 ± 0.81 | 0.48 | 182 | |
Aspirin (AA) | 180.16 | 232 | 3.5 | 1.19 ± 0.23 | 2.93 | 64 |
AA Adsorption | TC Adsorption | |
---|---|---|
ACC | Apatite | |
Qads (mmol/g) | 2.07 ± 0.06 | 0.06 ± 0.01 |
ACC/CDA | ||
Qads (mmol/g) | 2.10 ± 0.10 | 0.07 ± 0.01 |
Drug Loading | Live/Dead Assay | ||||
---|---|---|---|---|---|
Qads (µmol/g) | Qads (µg) | Live Cells/mm2 | Dead Cells/mm2 | “r” (L/D) | |
ACC/CDA | - | - | 18 ± 4 | 14 ± 2 | 1.30 |
ACC/CDA-TC | 133 ± 36 | 500 ± 130 | 18 ± 9 | 6 ± 2 | 3.00 |
ACC/CDA-AA | 134 ± 23 | 200 ± 30 | 51 ± 13 | 14 ± 2 | 3.65 |
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Olivier, F.; Bonnamy, S.; Rochet, N.; Drouet, C. Activated Carbon Fiber Cloth/Biomimetic Apatite: A Dual Drug Delivery System. Int. J. Mol. Sci. 2021, 22, 12247. https://doi.org/10.3390/ijms222212247
Olivier F, Bonnamy S, Rochet N, Drouet C. Activated Carbon Fiber Cloth/Biomimetic Apatite: A Dual Drug Delivery System. International Journal of Molecular Sciences. 2021; 22(22):12247. https://doi.org/10.3390/ijms222212247
Chicago/Turabian StyleOlivier, Florian, Sylvie Bonnamy, Nathalie Rochet, and Christophe Drouet. 2021. "Activated Carbon Fiber Cloth/Biomimetic Apatite: A Dual Drug Delivery System" International Journal of Molecular Sciences 22, no. 22: 12247. https://doi.org/10.3390/ijms222212247