Lung and Gut Microbiota Changes Associated with Pseudomonas aeruginosa Infection in Mouse Models of Cystic Fibrosis
Abstract
:1. Introduction
2. Results
2.1. P. aeruginosa Infection Influences Murine Health Both in KO and WT Mice
2.2. Microbiota Diversity Is Influenced by CFTR Genotype in the Gut and P. aeruginosa Infection in the Lung
2.3. Lung Microbiota Composition Is Strongly Influenced by P. aeruginosa Infection
2.4. Gut Microbiota Composition of KO Mice, but Not WT Mice, Is Influenced by P. aeruginosa Infection
3. Discussion
4. Materials and Methods
4.1. Ethic Statement
4.2. Mouse Model and P. aeruginosa Chronic Infection
4.3. DNA Extraction and Sequencing
4.4. Bioinformatic and Statistical Analyses
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gut | Stool | Lung | |
---|---|---|---|
WT (empty beads) | 37.26 ± 12.78 | 50.43 ± 24.48 | 33.45 ± 31.63 |
WT (RP73 infected) | 41.52 ± 20.21 | 30.88 ± 21.48 | 15.02 ± 7.81 |
KO (empty beads) | 9.81 ± 10.93 | 16.73 ± 10.69 | 40.69 ± 28.24 |
KO (RP73 infected) | 19.12 ± 9.93 | 18.44 ± 5.79 | 15.21 ± 7.90 |
Gut | Stool | Lung | |||||
---|---|---|---|---|---|---|---|
Pr(>F) | R2 | Pr(>F) | R2 | Pr(>F) | R2 | ||
(a) | Condition (RP73 infected—control) | 0.006 ** | 0.04305 | 0.026 * | 0.03572 | 0.001 *** | 0.09996 |
Genotype (KO—WT) | 0.001 *** | 0.13225 | 0.001 *** | 0.17754 | 0.894 | 0.01035 | |
(b) | WT (RP73 infected—control) | 0.098 | 0.04679 | 0.097 | 0.0461 | 0.023 * | 0.08876 |
KO (RP73 infected—control) | 0.002 ** | 0.16172 | 0.100 | 0.0819 | 0.007 ** | 0.17221 |
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Bacci, G.; Rossi, A.; Armanini, F.; Cangioli, L.; De Fino, I.; Segata, N.; Mengoni, A.; Bragonzi, A.; Bevivino, A. Lung and Gut Microbiota Changes Associated with Pseudomonas aeruginosa Infection in Mouse Models of Cystic Fibrosis. Int. J. Mol. Sci. 2021, 22, 12169. https://doi.org/10.3390/ijms222212169
Bacci G, Rossi A, Armanini F, Cangioli L, De Fino I, Segata N, Mengoni A, Bragonzi A, Bevivino A. Lung and Gut Microbiota Changes Associated with Pseudomonas aeruginosa Infection in Mouse Models of Cystic Fibrosis. International Journal of Molecular Sciences. 2021; 22(22):12169. https://doi.org/10.3390/ijms222212169
Chicago/Turabian StyleBacci, Giovanni, Alice Rossi, Federica Armanini, Lisa Cangioli, Ida De Fino, Nicola Segata, Alessio Mengoni, Alessandra Bragonzi, and Annamaria Bevivino. 2021. "Lung and Gut Microbiota Changes Associated with Pseudomonas aeruginosa Infection in Mouse Models of Cystic Fibrosis" International Journal of Molecular Sciences 22, no. 22: 12169. https://doi.org/10.3390/ijms222212169