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Article

High Resolution Structure of the Mature Capsid of Ralstonia solanacearum Bacteriophage ϕRSA1 by Cryo-Electron Microscopy

1
CEA, CNRS, IBS, Université Grenoble Alpes, F-38000 Grenoble, France
2
Center for Food Science and Wellness, Gunma University 4-2, Aramaki, Maebashi, Gunma 371-8510, Japan
3
Unit of Biotechnology, Division of Biological and Life Sciences, Graduate School of Integrated Science for Life, Hiroshima University, Higashi-Hiroshima 739-8530, Japan
4
Hiroshima Study Center, The Open University of Japan, Hiroshima 730-0053, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Alicja Wegrzyn
Int. J. Mol. Sci. 2021, 22(20), 11053; https://doi.org/10.3390/ijms222011053
Received: 9 September 2021 / Revised: 8 October 2021 / Accepted: 9 October 2021 / Published: 13 October 2021
(This article belongs to the Special Issue Bacteriophage—Molecular Studies 3.0)
The ϕRSA1 bacteriophage has been isolated from Ralstonia solanacearum, a gram negative bacteria having a significant economic impact on many important crops. We solved the three-dimensional structure of the ϕRSA1 mature capsid to 3.9 Å resolution by cryo-electron microscopy. The capsid shell, that contains the 39 kbp of dsDNA genome, has an icosahedral symmetry characterized by an unusual triangulation number of T = 7, dextro. The ϕRSA1 capsid is composed solely of the polymerization of the major capsid protein, gp8, which exhibits the typical “Johnson” fold first characterized in E. coli bacteriophage HK97. As opposed to the latter, the ϕRSA1 mature capsid is not stabilized by covalent crosslinking between its subunits, nor by the addition of a decoration protein. We further describe the molecular interactions occurring between the subunits of the ϕRSA1 capsid and their relationships with the other known bacteriophages. View Full-Text
Keywords: bacteriophage; structure; capsid; near atomic; electron microscopy bacteriophage; structure; capsid; near atomic; electron microscopy
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MDPI and ACS Style

Effantin, G.; Fujiwara, A.; Kawasaki, T.; Yamada, T.; Schoehn, G. High Resolution Structure of the Mature Capsid of Ralstonia solanacearum Bacteriophage ϕRSA1 by Cryo-Electron Microscopy. Int. J. Mol. Sci. 2021, 22, 11053. https://doi.org/10.3390/ijms222011053

AMA Style

Effantin G, Fujiwara A, Kawasaki T, Yamada T, Schoehn G. High Resolution Structure of the Mature Capsid of Ralstonia solanacearum Bacteriophage ϕRSA1 by Cryo-Electron Microscopy. International Journal of Molecular Sciences. 2021; 22(20):11053. https://doi.org/10.3390/ijms222011053

Chicago/Turabian Style

Effantin, Grégory, Akiko Fujiwara, Takeru Kawasaki, Takashi Yamada, and Guy Schoehn. 2021. "High Resolution Structure of the Mature Capsid of Ralstonia solanacearum Bacteriophage ϕRSA1 by Cryo-Electron Microscopy" International Journal of Molecular Sciences 22, no. 20: 11053. https://doi.org/10.3390/ijms222011053

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