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Pannexins and Connexins: Their Relevance for Oocyte Developmental Competence
 
 
Article

Connexin Expression Is Altered in Liver Development of Yotari (dab1 -/-) Mice

1
Mediterranean Institute for Life Sciences (MedILS), Meštrovićevo šetalište 45, 21000 Split, Croatia
2
Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Šoltanska 2, 21000 Split, Croatia
3
Department of Anatomy, Shiga University of Medical Science, Otsu 520-2192, Japan
4
Department of Medical Genetics, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and Herzegovina
*
Author to whom correspondence should be addressed.
Academic Editor: Mathieu Vinken
Int. J. Mol. Sci. 2021, 22(19), 10712; https://doi.org/10.3390/ijms221910712
Received: 30 August 2021 / Revised: 26 September 2021 / Accepted: 29 September 2021 / Published: 2 October 2021
(This article belongs to the Special Issue Connexin and Pannexin Signaling in Health and Disease 2.0)
Disabled-1 (Dab1) protein is an intracellular adaptor of reelin signaling required for prenatal neuronal migration, as well as postnatal neurotransmission, memory formation and synaptic plasticity. Yotari, an autosomal recessive mutant of the mouse Dab1 gene is recognizable by its premature death, unstable gait and tremor. Previous findings are mostly based on neuronal abnormalities caused by Dab1 deficiency, but the role of the reelin signaling pathway in nonneuronal tissues and organs has not been studied until recently. Hepatocytes, the most abundant cells in the liver, communicate via gap junctions (GJ) are composed of connexins. Cell communication disruption in yotari mice was examined by analyzing the expression of connexins (Cxs): Cx26, Cx32, Cx37, Cx40, Cx43 and Cx45 during liver development at 13.5 and 15.5 gestation days (E13.5 and E15.5). Analyses were performed using immunohistochemistry and fluorescent microscopy, followed by quantification of area percentage covered by positive signal. Data are expressed as a mean ± SD and analyzed by one-way ANOVA. All Cxs examined displayed a significant decrease in yotari compared to wild type (wt) individuals at E13.5. Looking at E15.5 we have similar results with exception of Cx37 showing negligible expression in wt. Channels formation triggered by pathological stimuli, as well as propensity to apoptosis, was studied by measuring the expression of Pannexin1 (Panx1) and Apoptosis-inducing factor (AIF) through developmental stages mentioned above. An increase in Panx1 expression of E15.5 yotari mice, as well as a strong jump of AIF in both phases suggesting that yotari mice are more prone to apoptosis. Our results emphasize the importance of gap junction intercellular communication (GJIC) during liver development and their possible involvement in liver pathology and diagnostics where they can serve as potential biomarkers and drug targets. View Full-Text
Keywords: gap junction; liver development; Cx26; Cx32; Cx37; Cx40; Cx43; Cx45; Panx1; AIF; yotari gap junction; liver development; Cx26; Cx32; Cx37; Cx40; Cx43; Cx45; Panx1; AIF; yotari
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MDPI and ACS Style

Paštar, V.; Lozić, M.; Kelam, N.; Filipović, N.; Bernard, B.; Katsuyama, Y.; Vukojević, K. Connexin Expression Is Altered in Liver Development of Yotari (dab1 -/-) Mice. Int. J. Mol. Sci. 2021, 22, 10712. https://doi.org/10.3390/ijms221910712

AMA Style

Paštar V, Lozić M, Kelam N, Filipović N, Bernard B, Katsuyama Y, Vukojević K. Connexin Expression Is Altered in Liver Development of Yotari (dab1 -/-) Mice. International Journal of Molecular Sciences. 2021; 22(19):10712. https://doi.org/10.3390/ijms221910712

Chicago/Turabian Style

Paštar, Vlatka, Mirela Lozić, Nela Kelam, Natalija Filipović, Branka Bernard, Yu Katsuyama, and Katarina Vukojević. 2021. "Connexin Expression Is Altered in Liver Development of Yotari (dab1 -/-) Mice" International Journal of Molecular Sciences 22, no. 19: 10712. https://doi.org/10.3390/ijms221910712

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