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Article

FluCell-SELEX Aptamers as Specific Binding Molecules for Diagnostics of the Health Relevant Gut Bacterium Akkermansia muciniphila

1
Institute for Pharmaceutical Biotechnology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany
2
Max Planck Institute for Polymer Research Mainz, Ackermannweg 10, 55128 Mainz, Germany
3
Institute for Medical Microbiology and Hygiene, University Hospital Ulm, 89081 Ulm, Germany
4
Department of General and Visceral Surgery, Surgery Center, Ulm University, Albert-Einstein-Allee 23, 89081 Ulm, Germany
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Filomena Sica
Int. J. Mol. Sci. 2021, 22(19), 10425; https://doi.org/10.3390/ijms221910425
Received: 30 August 2021 / Revised: 22 September 2021 / Accepted: 22 September 2021 / Published: 27 September 2021
(This article belongs to the Special Issue Aptamers: Functional-Structural Studies and Biomedical Applications)
Based on their unique properties, oligonucleotide aptamers have been named a gift of biological chemistry to life science. We report the development of DNA aptamers as the first high-affinity binding molecules available for fast and rapid labeling of the human gut bacterium Akkermansia muciniphila with a certain impact on Alzheimer´s disease. Fast and reliable analyses of the composition of microbiomes is an emerging field in microbiology. We describe the molecular evolution and biochemical characterization of a specific aptamer library by a FluCell-SELEX and the characterization of specific molecules from the library by bioinformatics. The aptamer AKK13.1 exerted universal applicability in different analysis techniques in modern microbiology, including fluorimetry, confocal laser scanning microscopy and flow cytometry. It was also functional as a specific binding entity hybridized to anchor primers chemically coupled via acrydite-modification to the surface of a polyacrylamide-hydrogel, which can be prototypically used for the construction of affinity surfaces in sensor chips. Together, the performance and methodological flexibility of the aptamers presented here may open new routes not only to develop novel Akkermansia-specific assays for clinical microbiology and the analyses of human stool samples but may also be an excellent starting point for the construction of novel electronic biosensors. View Full-Text
Keywords: aptamers; biosensors; FluCell-SELEX aptamers; biosensors; FluCell-SELEX
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MDPI and ACS Style

Raber, H.F.; Kubiczek, D.H.; Bodenberger, N.; Kissmann, A.-K.; D’souza, D.; Xing, H.; Mayer, D.; Xu, P.; Knippschild, U.; Spellerberg, B.; Weil, T.; Rosenau, F. FluCell-SELEX Aptamers as Specific Binding Molecules for Diagnostics of the Health Relevant Gut Bacterium Akkermansia muciniphila. Int. J. Mol. Sci. 2021, 22, 10425. https://doi.org/10.3390/ijms221910425

AMA Style

Raber HF, Kubiczek DH, Bodenberger N, Kissmann A-K, D’souza D, Xing H, Mayer D, Xu P, Knippschild U, Spellerberg B, Weil T, Rosenau F. FluCell-SELEX Aptamers as Specific Binding Molecules for Diagnostics of the Health Relevant Gut Bacterium Akkermansia muciniphila. International Journal of Molecular Sciences. 2021; 22(19):10425. https://doi.org/10.3390/ijms221910425

Chicago/Turabian Style

Raber, Heinz Fabian, Dennis Horst Kubiczek, Nicholas Bodenberger, Ann-Kathrin Kissmann, Deena D’souza, Hu Xing, Daniel Mayer, Pengfei Xu, Uwe Knippschild, Barbara Spellerberg, Tanja Weil, and Frank Rosenau. 2021. "FluCell-SELEX Aptamers as Specific Binding Molecules for Diagnostics of the Health Relevant Gut Bacterium Akkermansia muciniphila" International Journal of Molecular Sciences 22, no. 19: 10425. https://doi.org/10.3390/ijms221910425

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