C5a Activates a Pro-Inflammatory Gene Expression Profile in Human Gaucher iPSC-Derived Macrophages
Abstract
:1. Introduction
2. Results and Discussion
2.1. Recombinant C5a Induces an Elevated TNF-α-Mediated Inflammatory Response in GD Macrophages
2.2. Microarray Analysis of GD Macrophages
2.3. Differentially Expressed Genes between Untreated GD vs. Control Macrophages
2.3.1. Redox Imbalance
2.3.2. Gene Array Analysis Indicates Dysregulated Cholesterol Metabolism in GD
2.3.3. Gene Array Analysis Suggests Potential New Biomarkers for GD
2.4. rC5a-Stimulated GD vs. rC5a-Stimulated Control Macrophages
2.4.1. Pathway Analysis
2.4.2. C5a Treatment of GD Macrophages Upregulates Genes Involved in Hepatic Fibrosis
2.4.3. Inflammatory Gene Expression Profile Induced by rC5a in GD Macrophages
2.4.4. Wnt Signaling
3. Materials and Methods
3.1. iPSC Lines and Directed Differentiation to Macrophages
3.2. Flow Cytometry
3.3. Measurement of Cytokine Expression by qRT-PCR
3.4. Measurement of Cytokine Secretion by ELISA
3.5. Microarray Analysis
3.6. Phagocytosis Assays
3.6.1. Fcγ-Mediated Phagocytosis
3.6.2. Complement Receptor-Mediated Phagocytosis
3.7. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Serfecz, J.C.; Saadin, A.; Santiago, C.P.; Zhang, Y.; Bentzen, S.M.; Vogel, S.N.; Feldman, R.A. C5a Activates a Pro-Inflammatory Gene Expression Profile in Human Gaucher iPSC-Derived Macrophages. Int. J. Mol. Sci. 2021, 22, 9912. https://doi.org/10.3390/ijms22189912
Serfecz JC, Saadin A, Santiago CP, Zhang Y, Bentzen SM, Vogel SN, Feldman RA. C5a Activates a Pro-Inflammatory Gene Expression Profile in Human Gaucher iPSC-Derived Macrophages. International Journal of Molecular Sciences. 2021; 22(18):9912. https://doi.org/10.3390/ijms22189912
Chicago/Turabian StyleSerfecz, Jacquelyn C., Afsoon Saadin, Clayton P. Santiago, Yuji Zhang, Søren M. Bentzen, Stefanie N. Vogel, and Ricardo A. Feldman. 2021. "C5a Activates a Pro-Inflammatory Gene Expression Profile in Human Gaucher iPSC-Derived Macrophages" International Journal of Molecular Sciences 22, no. 18: 9912. https://doi.org/10.3390/ijms22189912