The Knockout of Enterobactin-Related Gene in Pectobacterium atrosepticum Results in Reduced Stress Resistance and Virulence towards the Primed Plants
Abstract
:1. Introduction
2. Results
2.1. Primary Characteristics of Enterobactin-Deficient Pba Mutant
2.2. Virulence of ΔentA Mutant
2.3. Stress Resistance of ΔentA Mutant
2.4. Virulence of ΔentA Mutant towards Salicylic Acid-Primed Plants
2.5. H2O2-Level in Infected and Non-Infected Tobacco Plants Pretreated or Not with Salicylic Acid
3. Discussion
4. Materials and Methods
4.1. Bacterial Strains, Media and Culture Conditions
4.2. Construction of entA Deletion Mutant
4.3. Construction of the Complementation ΔentA Mutant Strain Carring the entA Gene within the Plasmid
4.4. Plant Cultivation and Infection
4.5. Stress Tolerance Assay
4.6. Enzymatic Activity Assays
4.7. Gene Expression Analysis
4.8. Determination of the Hydrogen Peroxide Level
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gorshkov, V.; Parfirova, O.; Petrova, O.; Gogoleva, N.; Kovtunov, E.; Vorob’ev, V.; Gogolev, Y. The Knockout of Enterobactin-Related Gene in Pectobacterium atrosepticum Results in Reduced Stress Resistance and Virulence towards the Primed Plants. Int. J. Mol. Sci. 2021, 22, 9594. https://doi.org/10.3390/ijms22179594
Gorshkov V, Parfirova O, Petrova O, Gogoleva N, Kovtunov E, Vorob’ev V, Gogolev Y. The Knockout of Enterobactin-Related Gene in Pectobacterium atrosepticum Results in Reduced Stress Resistance and Virulence towards the Primed Plants. International Journal of Molecular Sciences. 2021; 22(17):9594. https://doi.org/10.3390/ijms22179594
Chicago/Turabian StyleGorshkov, Vladimir, Olga Parfirova, Olga Petrova, Natalia Gogoleva, Evgeny Kovtunov, Vladimir Vorob’ev, and Yuri Gogolev. 2021. "The Knockout of Enterobactin-Related Gene in Pectobacterium atrosepticum Results in Reduced Stress Resistance and Virulence towards the Primed Plants" International Journal of Molecular Sciences 22, no. 17: 9594. https://doi.org/10.3390/ijms22179594