Supramolecular Gels Incorporating Cordyline terminalis Leaf Extract as a Polyphenol Release Scaffold for Biomedical Applications
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preliminary Screening of Phytochemicals
2.2. Hydrogel Formation and Characterization
2.3. Polyphenol Release
2.4. Antioxidant Test
2.5. Cell Study
3. Materials and Methods
3.1. Materials
3.2. Aqueous Extract Preparation and Preliminary Screening of Phytochemicals
3.2.1. Aqueous Extract Preparation
3.2.2. Preliminary Screening of Phytochemicals
3.3. Polymer Synthesis
3.4. Hydrogel Formation and Characterization
3.5. Polyphenol Release
3.6. Antioxidant Test
3.7. Cell Study
3.8. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Phytochemicals | Method | Observation | Result |
---|---|---|---|
Alkaloids | Wagner test | Brown precipitation | + |
Cardiac glycosides | Keller-Kiliani Test | Red interface between two layers | + |
Coumarin glycosides | Alkaline test | Brown fluorescence | + |
Saponins | Foam test | A stable froth | + |
Phenolic compounds | FeCl3 test | Green dark color | + |
Steroids | Liebermann-Burchard test | None | − |
Phytosterol | Glacial acetic acid | None | − |
Hydrogels | Gelation Time (min) | Hydrogels | Gelation Time (min) |
---|---|---|---|
GPT3.75α-CD3.75 (GPT:α-CD = 1:1) | No gel | GPT’3.75α-CD7.5 (GPT’:α-CD = 1:2) | No gel |
GPT3.75α-CD1.875 (GPT:α-CD = 2:1) | No gel | GPT’3.75α-CD11.25 (GPT’:α-CD = 1:3) | No gel |
GPT3.75α-CD7.5 (GPT:α-CD = 1:2) | 135.6 8.1 | GPT3.75α-CD10.5aqCT3.1 | 85.1 9.4 |
GPT3.75α-CD9 (GPT:α-CD = 1:2.4) | 100.6 9.1 | GPT3.75α-CD10.5 aqCT6.2 | 101.9 11.2 |
GPT3.75α-CD9.75 (GPT:α-CD = 1:2.6) | 60.4 6.3 | GPT3.75α-CD10.5 aqCT12.5 | 297.7 4.3 |
GPT3.75α-CD10.5 (GPT:α-CD = 1:2.8) | 55.5 6.1 | GPT3.75α-CD15 aqCT9.4 | 51.1 5.1 |
GPT3.75α-CD11.25 (GPT:α-CD = 1:3) | 53.8 5.2 | GPT3.75α-CD15 aqCT12.5 | 59.6 8.7 |
GPT3.75α-CD15 (GPT:α-CD = 1:4) | 40.7 10.2 | GPT3.75α-CD15 aqCT15.6 | 190.5 5.3 |
GPT3.75α-CD9.45β-CD1.05 | 78.8 ± 13.5 | - | - |
Hydrogel Code | GPT 20 wt% (μL) | α-CD 14 wt% (μL) | α-CD 20 wt% (μL) | DIW (μL) | AqCT (g/mL, (μL) |
---|---|---|---|---|---|
GPT3.75α-CD10.5 | 75 | 300 | - | 25 | - |
GPT3.75α-CD10.5aqCT3.1 | 75 | 300 | - | - | 0.05, 25 |
GPT3.75α-CD10.5 aqCT6.2 | 75 | 300 | - | - | 0.10, 25 |
GPT3.75α-CD10.5 aqCT12.5 | 75 | 300 | - | - | 0.25, 25 |
GPT3.75α-CD15 aqCT9.4 | 75 | - | 300 | - | 0.15, 25 |
GPT3.75α-CD15 aqCT12.5 | 75 | - | 300 | - | 0.20, 25 |
GPT3.75α-CD15 aqCT15.6 | 75 | - | 300 | - | 0.25, 25 |
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Nguyen Thi, D.P.; Tran, D.L.; Le Thi, P.; Park, K.D.; Hoang Thi, T.T. Supramolecular Gels Incorporating Cordyline terminalis Leaf Extract as a Polyphenol Release Scaffold for Biomedical Applications. Int. J. Mol. Sci. 2021, 22, 8759. https://doi.org/10.3390/ijms22168759
Nguyen Thi DP, Tran DL, Le Thi P, Park KD, Hoang Thi TT. Supramolecular Gels Incorporating Cordyline terminalis Leaf Extract as a Polyphenol Release Scaffold for Biomedical Applications. International Journal of Molecular Sciences. 2021; 22(16):8759. https://doi.org/10.3390/ijms22168759
Chicago/Turabian StyleNguyen Thi, Dieu Phuong, Dieu Linh Tran, Phuong Le Thi, Ki Dong Park, and Thai Thanh Hoang Thi. 2021. "Supramolecular Gels Incorporating Cordyline terminalis Leaf Extract as a Polyphenol Release Scaffold for Biomedical Applications" International Journal of Molecular Sciences 22, no. 16: 8759. https://doi.org/10.3390/ijms22168759