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Article

Occurrence of Calcium Oscillations in Human Spermatozoa Is Based on Spatial Signaling Enzymes Distribution

1
Center for Theoretical Problems of Physico-Chemical Pharmacology, Russian Academy of Sciences, 30 Srednyaya Kalitnikovskaya, 109029 Moscow, Russia
2
National Medical Research Center of Pediatric Hematology, Oncology and Immunology Dmitry Rogachev, 1 Samory Mashela St., 117198 Moscow, Russia
3
Faculty of Physics, Lomonosov Moscow State University, 1/2 Leninskie, 119991 Moscow, Russia
4
AltraVita IVF and Gynecology Clinic, 4A Nagornaya, 117186 Moscow, Russia
5
Department of Normal Physiology, Sechenov First Moscow State Medical University, 8/2 Trubetskaya St., 119991 Moscow, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Manabu Yoshida
Int. J. Mol. Sci. 2021, 22(15), 8018; https://doi.org/10.3390/ijms22158018
Received: 14 June 2021 / Revised: 15 July 2021 / Accepted: 21 July 2021 / Published: 27 July 2021
(This article belongs to the Special Issue Molecular Mechanisms of Sperm Activation)
In human spermatozoa, calcium dynamics control most of fertilization events. Progesterone, present in the female reproductive system, can trigger several types of calcium responses, such as low-frequency oscillations. Here we aimed to identify the mechanisms of progesterone-induced calcium signaling in human spermatozoa. Progesterone-induced activation of fluorophore-loaded spermatozoa was studied by fluorescent microscopy. Two computational models were developed to describe the spermatozoa calcium responses: a homogeneous one based on a system of ordinary differential equations and a three-dimensional one with added space dimensions and diffusion for the cytosolic species. In response to progesterone, three types of calcium responses were observed in human spermatozoa: a single transient rise of calcium concentration in cytosol, a steady elevation, or low-frequency oscillations. The homogenous model provided qualitative description of the oscillatory and the single spike responses, while the three-dimensional model captured the calcium peak shape and the frequency of calcium oscillations. The model analysis demonstrated that an increase in the calcium diffusion coefficient resulted in the disappearance of the calcium oscillations. Additionally, in silico analysis suggested that the spatial distribution of calcium signaling enzymes governs the appearance of calcium oscillations in progesterone-activated human spermatozoa. View Full-Text
Keywords: CatSper; sperm cell; calcium signaling; progesterone; computational modeling; reaction-diffusion system CatSper; sperm cell; calcium signaling; progesterone; computational modeling; reaction-diffusion system
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MDPI and ACS Style

Korobkin, J.; Balabin, F.A.; Yakovenko, S.A.; Simonenko, E.Y.; Sveshnikova, A.N. Occurrence of Calcium Oscillations in Human Spermatozoa Is Based on Spatial Signaling Enzymes Distribution. Int. J. Mol. Sci. 2021, 22, 8018. https://doi.org/10.3390/ijms22158018

AMA Style

Korobkin J, Balabin FA, Yakovenko SA, Simonenko EY, Sveshnikova AN. Occurrence of Calcium Oscillations in Human Spermatozoa Is Based on Spatial Signaling Enzymes Distribution. International Journal of Molecular Sciences. 2021; 22(15):8018. https://doi.org/10.3390/ijms22158018

Chicago/Turabian Style

Korobkin, Julia, Fedor A. Balabin, Sergey A. Yakovenko, Ekaterina Yu. Simonenko, and Anastasia N. Sveshnikova. 2021. "Occurrence of Calcium Oscillations in Human Spermatozoa Is Based on Spatial Signaling Enzymes Distribution" International Journal of Molecular Sciences 22, no. 15: 8018. https://doi.org/10.3390/ijms22158018

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