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Plant Parasites under Pressure: Effects of Abiotic Stress on the Interactions between Parasitic Plants and Their Hosts
Article

Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress

1
Department for Molecular Biology, Ruđer Bošković Institute, Bijenička Cesta 54, 10000 Zagreb, Croatia
2
Department for Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102, 10000 Zagreb, Croatia
3
Laboratory of Growth Regulators, Faculty of Science of Palacký University & Institute of Experimental Botany of the Czech Academy of Sciences, Šlechtitelů 27, CZ-783 71 Olomouc, Czech Republic
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Richard R.-C. Wang
Int. J. Mol. Sci. 2021, 22(15), 7993; https://doi.org/10.3390/ijms22157993
Received: 16 May 2021 / Revised: 19 July 2021 / Accepted: 19 July 2021 / Published: 27 July 2021
(This article belongs to the Special Issue Mapping Abiotic Stress-Tolerance Genes in Plants 2021)
Salt and osmotic stress are the main abiotic stress factors affecting plant root growth and architecture. We investigated the effect of salt (100 mM NaCl) and osmotic (200 mM mannitol) stress on the auxin metabolome by UHPLC-MS/MS, auxin distribution by confocal microscopy, and transcript levels of selected genes by qRT-PCR in Arabidopsis thaliana ecotype Columbia-0 (Col-0) and DR5rev::GFP (DR5) line. During long-term stress (13 days), a stability of the auxin metabolome and a tendency to increase indole-3-acetic acid (IAA) were observed, especially during salt stress. Short-term stress (3 h) caused significant changes in the auxin metabolome, especially NaCl treatment resulted in a significant reduction of IAA. The data derived from auxin profiling were consistent with gene expressions showing the most striking changes in the transcripts of YUC, GH3, and UGT transcripts, suggesting disruption of auxin biosynthesis, but especially in the processes of amide and ester conjugation. These data were consistent with the auxin distribution observed in the DR5 line. Moreover, NaCl treatment caused a redistribution of auxin signals from the quiescent center and the inner layers of the root cap to the epidermal and cortical cells of the root elongation zone. The distribution of PIN proteins was also disrupted by salt stress; in particular, PIN2 was suppressed, even after 5 min of treatment. Based on our results, the DR5 line was more sensitive to the applied stresses than Col-0, although both lines showed similar trends in root morphology, as well as transcriptome and metabolome parameters under stress conditions. View Full-Text
Keywords: abiotic stress; Arabidopsis thaliana; auxin distribution; auxin metabolome; auxin transcriptome; root growth abiotic stress; Arabidopsis thaliana; auxin distribution; auxin metabolome; auxin transcriptome; root growth
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MDPI and ACS Style

Smolko, A.; Bauer, N.; Pavlović, I.; Pěnčík, A.; Novák, O.; Salopek-Sondi, B. Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress. Int. J. Mol. Sci. 2021, 22, 7993. https://doi.org/10.3390/ijms22157993

AMA Style

Smolko A, Bauer N, Pavlović I, Pěnčík A, Novák O, Salopek-Sondi B. Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress. International Journal of Molecular Sciences. 2021; 22(15):7993. https://doi.org/10.3390/ijms22157993

Chicago/Turabian Style

Smolko, Ana, Nataša Bauer, Iva Pavlović, Aleš Pěnčík, Ondřej Novák, and Branka Salopek-Sondi. 2021. "Altered Root Growth, Auxin Metabolism and Distribution in Arabidopsis thaliana Exposed to Salt and Osmotic Stress" International Journal of Molecular Sciences 22, no. 15: 7993. https://doi.org/10.3390/ijms22157993

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