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Article

Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years

1
Department of Genetic Toxicology and Epigenetics, Institute of Experimental Medicine CAS, Videnska 1083, 142 20 Prague 4, Czech Republic
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Department of Occupational Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Na Bojisti 1, 120 00 Prague 2, Czech Republic
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Department of Aerosol Chemistry and Physics, Institute of Chemical Process Fundamentals CAS, Rozvojova 1, 165 02 Prague 6, Czech Republic
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Experimental Medicine Centre, Institute for Clinical and Experimental Medicine, Videnska 1958/9, 140 21 Prague 4, Czech Republic
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Department of Machining and Assembly, Department of Engineering Technology, Department of Material Science, Faculty of Mechanical Engineering, Technical University in Liberec, Studentska 1402/2, 461 17 Liberec, Czech Republic
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Department of Nanotoxicology and Molecular Epidemiology, Institute of Experimental Medicine CAS, Videnska 1083, 142 20 Prague 4, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editors: Giovanna Della Porta and Antonietta Santoro
Int. J. Mol. Sci. 2021, 22(15), 7834; https://doi.org/10.3390/ijms22157834
Received: 25 April 2021 / Revised: 15 July 2021 / Accepted: 21 July 2021 / Published: 22 July 2021
(This article belongs to the Special Issue Epigenetic Effects and Toxicity of Environmental Pollutants)
A DNA methylation pattern represents an original plan of the function settings of individual cells and tissues. The basic strategies of its development and changes during the human lifetime are known, but the details related to its modification over the years on an individual basis have not yet been studied. Moreover, current evidence shows that environmental exposure could generate changes in DNA methylation settings and, subsequently, the function of genes. In this study, we analyzed the effect of chronic exposure to nanoparticles (NP) in occupationally exposed workers repeatedly sampled in four consecutive years (2016–2019). A detailed methylation pattern analysis of 14 persons (10 exposed and 4 controls) was performed on an individual basis. A microarray-based approach using chips, allowing the assessment of more than 850 K CpG loci, was used. Individual DNA methylation patterns were compared by principal component analysis (PCA). The results show the shift in DNA methylation patterns in individual years in all the exposed and control subjects. The overall range of differences varied between the years in individual persons. The differences between the first and last year of examination (a three-year time period) seem to be consistently greater in the NP-exposed subjects in comparison with the controls. The selected 14 most differently methylated cg loci were relatively stable in the chronically exposed subjects. In summary, the specific type of long-term exposure can contribute to the fixing of relevant epigenetic changes related to a specific environment as, e.g., NP inhalation. View Full-Text
Keywords: DNA methylation; epigenetics; human; time changes; microarrays; nanoparticles; occupational exposure DNA methylation; epigenetics; human; time changes; microarrays; nanoparticles; occupational exposure
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MDPI and ACS Style

Rossnerova, A.; Honkova, K.; Chvojkova, I.; Pelclova, D.; Zdimal, V.; Hubacek, J.A.; Lischkova, L.; Vlckova, S.; Ondracek, J.; Dvorackova, S.; Topinka, J.; Rossner, P., Jr. Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years. Int. J. Mol. Sci. 2021, 22, 7834. https://doi.org/10.3390/ijms22157834

AMA Style

Rossnerova A, Honkova K, Chvojkova I, Pelclova D, Zdimal V, Hubacek JA, Lischkova L, Vlckova S, Ondracek J, Dvorackova S, Topinka J, Rossner P Jr. Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years. International Journal of Molecular Sciences. 2021; 22(15):7834. https://doi.org/10.3390/ijms22157834

Chicago/Turabian Style

Rossnerova, Andrea, Katerina Honkova, Irena Chvojkova, Daniela Pelclova, Vladimir Zdimal, Jaroslav A. Hubacek, Lucie Lischkova, Stepanka Vlckova, Jakub Ondracek, Stepanka Dvorackova, Jan Topinka, and Pavel Rossner Jr. 2021. "Individual DNA Methylation Pattern Shifts in Nanoparticles-Exposed Workers Analyzed in Four Consecutive Years" International Journal of Molecular Sciences 22, no. 15: 7834. https://doi.org/10.3390/ijms22157834

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