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The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells

1
Virology Department, Croatian Veterinary Institute, Savska Cesta 143, 10 000 Zagreb, Croatia
2
Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia
3
Surgery, Orthopaedics and Ophthalmology Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia
4
Veterinary Clinic for Small Animals Buba, Dore Pfanove 11, 10 000 Zagreb, Croatia
5
Department of Microbiology and Infectious Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia
6
Department for Bacteriology and Parasitology, Croatian Veterinary Institute, Savska Cesta 143, 10 000 Zagreb, Croatia
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Maria Grazia Cifone
Int. J. Mol. Sci. 2021, 22(14), 7476; https://doi.org/10.3390/ijms22147476
Received: 8 June 2021 / Revised: 30 June 2021 / Accepted: 9 July 2021 / Published: 12 July 2021
(This article belongs to the Section Molecular Biology)
The influence of cultivation on the expression pattern of canine adipose-derived mesenchymal stem cells (cAD-MSCs) surface markers, contributing to, among others, the promotion of growth, proliferation, differentiation and immunomodulatory mechanisms of an excellent therapeutic, is still unknown. To fill the gap, we investigated CD90, CD44, CD73, CD29, CD271, CD105, CD45 and CD14 patterns of expression at the protein level with flow cytometry and mRNA level using a real-time polymerase chain reaction array. Gentle variations of expression occurred during cultivation, along with increased CD90, CD44 and CD29 expression, low and decreasing CD271 and CD73 expression and a decrease of initially high CD105. As expected, CD45 and CD14 were not expressed by cAD-MSCs. Interestingly, we discovered a significant decrease of CD73 expression, compared to early (P1–P3) to late (P4–P6) passages, although the CD73 gene expression was found to be stable. The percentage of positive cells was found to be higher for all positive markers up to P4. As CD73′s one important feature is a modulation from a pro-inflammatory environment to an anti-inflammatory milieu, the expression of CD73 in our conditions indicate the need to consider the time cells spend in vitro before being transplanted into patients, since it could impact their favourable therapeutical properties. View Full-Text
Keywords: stem cells; in vitro cultivation; immunophenotype; gene expression; flow cytometry; canine stem cells; in vitro cultivation; immunophenotype; gene expression; flow cytometry; canine
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MDPI and ACS Style

Krešić, N.; Prišlin, M.; Vlahović, D.; Kostešić, P.; Ljolje, I.; Brnić, D.; Turk, N.; Musulin, A.; Habrun, B. The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells. Int. J. Mol. Sci. 2021, 22, 7476. https://doi.org/10.3390/ijms22147476

AMA Style

Krešić N, Prišlin M, Vlahović D, Kostešić P, Ljolje I, Brnić D, Turk N, Musulin A, Habrun B. The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells. International Journal of Molecular Sciences. 2021; 22(14):7476. https://doi.org/10.3390/ijms22147476

Chicago/Turabian Style

Krešić, Nina, Marina Prišlin, Dunja Vlahović, Petar Kostešić, Ivana Ljolje, Dragan Brnić, Nenad Turk, Andrija Musulin, and Boris Habrun. 2021. "The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells" International Journal of Molecular Sciences 22, no. 14: 7476. https://doi.org/10.3390/ijms22147476

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