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Review

Best Practices and Progress in Precision-Cut Liver Slice Cultures

1
Liver Cell Biology Research Group, Department of Basic Biomedical Sciences, Vrije Universiteit Brussel, 1090 Brussels, Belgium
2
Department of Gastro-Enterology and Hepatology, Universitair Ziekenhuis Brussel, 1090 Brussels, Belgium
*
Author to whom correspondence should be addressed.
Academic Editor: Johannes Haybaeck
Int. J. Mol. Sci. 2021, 22(13), 7137; https://doi.org/10.3390/ijms22137137
Received: 31 May 2021 / Revised: 25 June 2021 / Accepted: 30 June 2021 / Published: 1 July 2021
(This article belongs to the Collection Molecular Mechanisms of Human Liver Diseases)
Thirty-five years ago, precision-cut liver slices (PCLS) were described as a promising tool and were expected to become the standard in vitro model to study liver disease as they tick off all characteristics of a good in vitro model. In contrast to most in vitro models, PCLS retain the complex 3D liver structures found in vivo, including cell–cell and cell–matrix interactions, and therefore should constitute the most reliable tool to model and to investigate pathways underlying chronic liver disease in vitro. Nevertheless, the biggest disadvantage of the model is the initiation of a procedure-induced fibrotic response. In this review, we describe the parameters and potential of PCLS cultures and discuss whether the initially described limitations and pitfalls have been overcome. We summarize the latest advances in PCLS research and critically evaluate PCLS use and progress since its invention in 1985. View Full-Text
Keywords: PCLS; chronic liver disease; 3D; in vitro liver PCLS; chronic liver disease; 3D; in vitro liver
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MDPI and ACS Style

Dewyse, L.; Reynaert, H.; van Grunsven, L.A. Best Practices and Progress in Precision-Cut Liver Slice Cultures. Int. J. Mol. Sci. 2021, 22, 7137. https://doi.org/10.3390/ijms22137137

AMA Style

Dewyse L, Reynaert H, van Grunsven LA. Best Practices and Progress in Precision-Cut Liver Slice Cultures. International Journal of Molecular Sciences. 2021; 22(13):7137. https://doi.org/10.3390/ijms22137137

Chicago/Turabian Style

Dewyse, Liza, Hendrik Reynaert, and Leo A. van Grunsven 2021. "Best Practices and Progress in Precision-Cut Liver Slice Cultures" International Journal of Molecular Sciences 22, no. 13: 7137. https://doi.org/10.3390/ijms22137137

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