Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner

Maślińska-Gromadka, Karolina; Barabasz, Anna; Palusińska, Małgorzata; Kozak, Katarzyna; Antosiewicz, Danuta Maria

doi:10.3390/ijms22105355

Open AccessArticle

Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner

by

Karolina Maślińska-Gromadka

^†,

Anna Barabasz

^†

,

Małgorzata Palusińska

,

Katarzyna Kozak

and

Danuta Maria Antosiewicz

^*

Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of Warsaw, 1 Miecznikowa Str., 02-096 Warszawa, Poland

^*

Author to whom correspondence should be addressed.

^†

Both authors contributed equally to the paper.

Int. J. Mol. Sci. 2021, 22(10), 5355; https://doi.org/10.3390/ijms22105355

Submission received: 22 March 2021 / Revised: 26 April 2021 / Accepted: 28 April 2021 / Published: 19 May 2021

(This article belongs to the Section Molecular Plant Sciences)

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Abstract

In tobacco, the efficiency of Zn translocation to shoots depends on Zn/Cd status. Previous studies pointed to the specific contribution of root parts in the regulation of this process, as well as the role of NtZIP4A/B (from the ZIP family; Zrt Irt-like Proteins). Here, to verify this hypothesis, NtZIP4A/B RNAi lines were generated. Then, in plants exposed to combinations of Zn and Cd concentrations in the medium, the consequences of NtZIP4A/B suppression for the translocation of both metals were determined. Furthermore, the apical, middle, and basal root parts were examined for accumulation of both metals, for Zn localization (using Zinpyr-1), and for modifications of the expression pattern of ZIP genes. Our results confirmed the role of NtZIP4A/B in the control of Zn/Cd-status-dependent transfer of both metals to shoots. Furthermore, they indicated that the middle and basal root parts contributed to the regulation of this process by acting as a reservoir for excess Zn and Cd. Expression studies identified several candidate ZIP genes that interact with NtZIP4A/B in the root in regulating Zn and Cd translocation to the shoot, primarily NtZIP1-like in the basal root part and NtZIP2 in the middle one.

Keywords: cadmium; GUS; NtZIP4; root-to-shoot translocation; tobacco; zinc; ZIP genes; Zinpyr-1

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MDPI and ACS Style

Maślińska-Gromadka, K.; Barabasz, A.; Palusińska, M.; Kozak, K.; Antosiewicz, D.M. Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner. Int. J. Mol. Sci. 2021, 22, 5355. https://doi.org/10.3390/ijms22105355

AMA Style

Maślińska-Gromadka K, Barabasz A, Palusińska M, Kozak K, Antosiewicz DM. Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner. International Journal of Molecular Sciences. 2021; 22(10):5355. https://doi.org/10.3390/ijms22105355

Chicago/Turabian Style

Maślińska-Gromadka, Karolina, Anna Barabasz, Małgorzata Palusińska, Katarzyna Kozak, and Danuta Maria Antosiewicz. 2021. "Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner" International Journal of Molecular Sciences 22, no. 10: 5355. https://doi.org/10.3390/ijms22105355

APA Style

Maślińska-Gromadka, K., Barabasz, A., Palusińska, M., Kozak, K., & Antosiewicz, D. M. (2021). Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner. International Journal of Molecular Sciences, 22(10), 5355. https://doi.org/10.3390/ijms22105355

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Suppression of NtZIP4A/B Changes Zn and Cd Root-to-Shoot Translocation in a Zn/Cd Status-Dependent Manner

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