The Prominin-1-Derived Peptide Improves Cardiac Function Following Ischemia
Abstract
:1. Introduction
2. Results
2.1. PR1P Improves Murine Myocardial Function Post-Infarction
2.2. PR1P Improves Rat Myocardial Function Post-Infarction
2.3. PR1P Stabilizes VEGF, Upregulates VEGF Signaling and Mitigates Apoptosis
3. Discussion
4. Material and Methods
4.1. Rodent Models of Myocardial Infarction
4.2. Hemodynamic Measurements in Mice
4.3. Echocardiographic Studies in Mice and Rats
4.4. Histology and Immunochemistry (Rat Heart Infarct Quantification)
4.5. Cell Culture
4.6. Apoptosis Assay
4.7. Western Blot Analysis
4.8. Reagents and Peptides
4.9. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Heart Rate (bpm) | MAX-LVP (mmHg) | MIN-LVP (mmHg) | EDM-LVP (mmHg) | MAX-LV_V (uL) | MIN-LV_V (uL) | |
SHAM | 452.8 ± 22.8 | 100.9 ± 4.9 | −3.4 ± 2 | 1.9 ± 0.6 | 49.2 ± 11.2 | 15.8 ± 5.9 |
SP | 468.9 ± 48.3 | 89.7 ± 6.3 | 7.9 ± 5.2 | 16 ± 6.4 | 53.2 ± 6.7 | 35.5 ± 6.5 |
PR1P | 483.5 ± 66.8 | 94.5 ± 8.3 | 5 ± 6.6 | 10.7 ± 7.6 | 45.4 ± 7.3 | 22.8 ± 6.8 |
SHAM vs. SP | 0.8149 | 0.0052 | 0.0005 | 0.0002 | 0.538 | 0.0001 |
SHAM vs. PR1P | 0.4778 | 0.1433 | 0.009 | 0.0203 | 0.5633 | 0.0986 |
PR1P vs. SP | 0.7215 | 0.1258 | 0.2954 | 0.0600 | 0.0151 | 0.0001 |
Stroke Volume (uL) | Stroke Work (mjoules) | dp/dt MAX (mmHg/Sec) | dp/dt MIN (mmHg/Sec) | Aortic Pressure MAX Systolic (mmHg/Sec) | Aortic Pressure MIN Diastolic (mmHg/Sec) | |
SHAM | 26.4 ± 5.9 | 2672 ± 719 | 8677 ± 751 | −7876 ± 919 | 100.2 ± 5.1 | 70.8 ± 7.5 |
SP | 12.8 ± 3.4 | 1066 ± 296 | 5999.7 ± 1031 | −4942 ± 765 | 90.2 ± 5.6 | 68.6 ± 5.5 |
PR1P | 17.3 ± 4.1 | 1493 ± 414 | 6817.7 ± 1518 | 5214.1 ± 1050 | 92.9 ± 7.9 | 71.9 ± 5.7 |
SHAM vs. SP | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0073 | 0.7283 |
SHAM vs. PR1P | 0.0001 | 0.0001 | 0.0078 | 0.0001 | 0.0586 | 0.9120 |
PR1P vs. SP | 0.0061 | 0.0159 | 0.1372 | 0.6534 | 0.4489 | 0.2415 |
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Adini, A.; Adini, I.; Grad, E.; Tal, Y.; Danenberg, H.D.; Kang, P.M.; Matthews, B.D.; D’Amato, R.J. The Prominin-1-Derived Peptide Improves Cardiac Function Following Ischemia. Int. J. Mol. Sci. 2021, 22, 5169. https://doi.org/10.3390/ijms22105169
Adini A, Adini I, Grad E, Tal Y, Danenberg HD, Kang PM, Matthews BD, D’Amato RJ. The Prominin-1-Derived Peptide Improves Cardiac Function Following Ischemia. International Journal of Molecular Sciences. 2021; 22(10):5169. https://doi.org/10.3390/ijms22105169
Chicago/Turabian StyleAdini, Avner, Irit Adini, Etty Grad, Yuval Tal, Haim D. Danenberg, Peter M. Kang, Benjamin D. Matthews, and Robert J. D’Amato. 2021. "The Prominin-1-Derived Peptide Improves Cardiac Function Following Ischemia" International Journal of Molecular Sciences 22, no. 10: 5169. https://doi.org/10.3390/ijms22105169