CK2 Down-Regulation Increases the Expression of Senescence-Associated Secretory Phenotype Factors through NF-κB Activation
Abstract
:1. Introduction
2. Results
2.1. CK2 Down-Regulation Stimulates the Expression of SASP Factors by Enhancing the Nuclear Localization of NF-κB in Human Cancer Cells
2.2. Activation of the AKT-IKK-IκB Pathway Is Associated with the CK2 Down-Regulation-Mediated Nuclear Import of NF-κB
2.3. SIRT1 Attenuates both RelA/p65 Acetylation and Activation of the AKT-IKK-IκB Axis Mediated by CK2 Down-Regulation
2.4. Triciribine and Resvzeratrol Attenuate the Expression of MMP Orthologs Induced by CK2 Down-Regulation in Nematodes
2.5. Antisense Inhibitors of miR-186, miR-216b, miR-337-3p, and miR-760 Attenuate SASP Gene Expression Induced by Lipopolysaccharide (LPS)
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture, RNAi, and DNA Transfection
4.3. Immunoblotting
4.4. RT-PCR
4.5. Isolation of Nuclear and Cytoplasmic Extracts
4.6. Culture of Nematodes and RNAi Experiment
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
CK2 | protein kinase CK2 |
HMG | high mobility group |
IκB | inhibitors of NF-κB |
IKK | IκB kinase |
IL | interleukin |
LPS | lipopolysaccharide |
mTOR | mammalian target of rapamycin |
MMP | matrix metalloproteinase |
NF-κB | nuclear factor-κB |
RT-PCR | reverse transcription-polymerase chain reaction |
PI3K | phosphatidylinositol 3-kinase |
ROS | reactive oxygen species |
SA-β-gal | senescence-associated β-galactosidase |
SAHF | senescence-associated heterochromatin foci |
SASP | senescence-associated secretory phenotype |
siRNA | small interfering RNA |
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Song, J.; Bae, Y.-S. CK2 Down-Regulation Increases the Expression of Senescence-Associated Secretory Phenotype Factors through NF-κB Activation. Int. J. Mol. Sci. 2021, 22, 406. https://doi.org/10.3390/ijms22010406
Song J, Bae Y-S. CK2 Down-Regulation Increases the Expression of Senescence-Associated Secretory Phenotype Factors through NF-κB Activation. International Journal of Molecular Sciences. 2021; 22(1):406. https://doi.org/10.3390/ijms22010406
Chicago/Turabian StyleSong, Junbin, and Young-Seuk Bae. 2021. "CK2 Down-Regulation Increases the Expression of Senescence-Associated Secretory Phenotype Factors through NF-κB Activation" International Journal of Molecular Sciences 22, no. 1: 406. https://doi.org/10.3390/ijms22010406