The Role of Gut Microbiome in Psoriasis: Oral Administration of Staphylococcus aureus and Streptococcus danieliae Exacerbates Skin Inflammation of Imiquimod-Induced Psoriasis-Like Dermatitis
Abstract
:1. Introduction
2. Results
2.1. Abundance of Staphylococcus aureus and Streptococcus danieliae in the Gut Microbiome of keratinocyte-specific caspase-1 transgenic (Kcasp1Tg) Mice
2.2. Oral Administration of SA and SD Worsened Skin Inflammation
2.3. The Expression of TNF-α, IL-17A, IL-17F, and IL-22 Was Elevated in the Skin of SA- and SD-Administered Mice
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. 16S rRNA Sequencing and Analysis of Gut Microbiome
4.3. Bacterial Samples
4.4. Experimentally Induced Psoriasis
4.5. Quantitative Real-Time PCR
4.6. Statistical Analysis
4.7. Histological Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
Kcasp1Tg | keratinocyte-specific caspase-1 transgenic mouse |
SA | Staphylococcus aureus |
SD | Streptococcus danieliae |
BU | Bacteroides uniformis |
CS | Clostridum scindens |
WT | Wild type |
CFU | Colony forming unit |
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Okada, K.; Matsushima, Y.; Mizutani, K.; Yamanaka, K. The Role of Gut Microbiome in Psoriasis: Oral Administration of Staphylococcus aureus and Streptococcus danieliae Exacerbates Skin Inflammation of Imiquimod-Induced Psoriasis-Like Dermatitis. Int. J. Mol. Sci. 2020, 21, 3303. https://doi.org/10.3390/ijms21093303
Okada K, Matsushima Y, Mizutani K, Yamanaka K. The Role of Gut Microbiome in Psoriasis: Oral Administration of Staphylococcus aureus and Streptococcus danieliae Exacerbates Skin Inflammation of Imiquimod-Induced Psoriasis-Like Dermatitis. International Journal of Molecular Sciences. 2020; 21(9):3303. https://doi.org/10.3390/ijms21093303
Chicago/Turabian StyleOkada, Karin, Yoshiaki Matsushima, Kento Mizutani, and Keiichi Yamanaka. 2020. "The Role of Gut Microbiome in Psoriasis: Oral Administration of Staphylococcus aureus and Streptococcus danieliae Exacerbates Skin Inflammation of Imiquimod-Induced Psoriasis-Like Dermatitis" International Journal of Molecular Sciences 21, no. 9: 3303. https://doi.org/10.3390/ijms21093303
APA StyleOkada, K., Matsushima, Y., Mizutani, K., & Yamanaka, K. (2020). The Role of Gut Microbiome in Psoriasis: Oral Administration of Staphylococcus aureus and Streptococcus danieliae Exacerbates Skin Inflammation of Imiquimod-Induced Psoriasis-Like Dermatitis. International Journal of Molecular Sciences, 21(9), 3303. https://doi.org/10.3390/ijms21093303