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Open AccessArticle

Biocatalytic CO2 Absorption and Structural Studies of Carbonic Anhydrase under Industrially-Relevant Conditions

1
LAQV-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal
2
Biotechnology Division, Research and Development Center, PETROBRAS, Av. Horácio Macedo, 950. Ilha do Fundão, Rio de Janeiro 21941-915, Brazil
3
UCIBIO-REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(8), 2918; https://doi.org/10.3390/ijms21082918
Received: 6 March 2020 / Revised: 10 April 2020 / Accepted: 15 April 2020 / Published: 22 April 2020
(This article belongs to the Special Issue Carbonic Anhydrases: A Superfamily of Ubiquitous Enzymes)
The unprecedently high CO2 levels in the atmosphere evoke the urgent need for development of technologies for mitigation of its emissions. Among the alternatives, the biocatalytic route has been claimed as one of the most promising. In the present work, the carbonic anhydrase from bovine erythrocytes (BCA) was employed as a model enzyme for structural studies in an aqueous phase at alkaline pH, which is typical of large-scale absorption processes under operation. Circular dichroism (CD) analysis revealed a high enzymatic stability at pH 10 with a prominent decrease of the melting temperature above this value. The CO2 absorption capacity of the aqueous solutions were assessed by online monitoring of pressure decay in a stainless-steel cell, which indicated a better performance at pH 10 with a kinetic rate increase of up to 43%, as compared to non-biocatalytic conditions. Even low enzyme concentrations (0.2 mg g−1) proved to be sufficient to improve the overall CO2 capture process performance. The enzyme-enhanced approach of CO2 capture presents a high potential and should be further studied. View Full-Text
Keywords: carbonic anhydrase; greenhouse gases; CO2 capture; circular dichroism; fluorescence carbonic anhydrase; greenhouse gases; CO2 capture; circular dichroism; fluorescence
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Castro, A.M.; Ferreira, E.; Portugal, C.; Neves, L.A.; Crespo, J.G. Biocatalytic CO2 Absorption and Structural Studies of Carbonic Anhydrase under Industrially-Relevant Conditions. Int. J. Mol. Sci. 2020, 21, 2918.

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