Identification of EIL and ERF Genes Related to Fruit Ripening in Peach
Abstract
:1. Introduction
2. Results
2.1. Identification of EIL and ERF Genes That Are Expressed in Peach Fruit
2.2. Expression Profile of EIL and ERF Genes in Peach Fruit
2.3. Roles of PpEILs and PpERFs in Transcriptional Regulation of Ethylene Biosynthesis Genes
2.4. Analysis of Transcriptional Activation Ability of PpERF Proteins in Yeast
2.5. Identification of Cis-Elements in the PpACS1 Promoter That Interact with EIL and ERF TFs
3. Discussion
4. Methods
4.1. Plant Material
4.2. Heatmap Analysis of the ERF Gene Family
4.3. RNA Extraction and Quantitative Real-Time PCR (qRT-PCR)
4.4. Dual Luciferase Reporter Assay
4.5. Measurement of Transcriptional Activation Activity of ERFs in Yeast
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene | Accession No. | Flower | Leaf | Fruit | |||||
---|---|---|---|---|---|---|---|---|---|
WF | RF | GL | RL | YS3 | RS3 | YS4 | RS4 | ||
PpEIL1 | Prupe.6G018200 | 13.2 | 13.0 | 13.5 | 15.6 | 12.5 | 15.6 | 15.3 | 21.6 |
PpEIL2 | Prupe.2G058400 | 78.8 | 77.7 | 130.8 | 146.3 | 198.6 | 171.7 | 167.9 | 225.2 |
PpEIL3 | Prupe.2G058500 | 42.9 | 35.8 | 68.9 | 68.9 | 61.5 | 44.0 | 46.8 | 65.5 |
PpEIL4 | Prupe.6G181600 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
PpEIL5 | Prupe.2G070300 | 14.8 | 13.4 | 19.2 | 23.1 | 22.2 | 22.6 | 8.1 | 30.6 |
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Zhou, H.; Zhao, L.; Yang, Q.; Amar, M.H.; Ogutu, C.; Peng, Q.; Liao, L.; Zhang, J.; Han, Y. Identification of EIL and ERF Genes Related to Fruit Ripening in Peach. Int. J. Mol. Sci. 2020, 21, 2846. https://doi.org/10.3390/ijms21082846
Zhou H, Zhao L, Yang Q, Amar MH, Ogutu C, Peng Q, Liao L, Zhang J, Han Y. Identification of EIL and ERF Genes Related to Fruit Ripening in Peach. International Journal of Molecular Sciences. 2020; 21(8):2846. https://doi.org/10.3390/ijms21082846
Chicago/Turabian StyleZhou, Hui, Lei Zhao, Qiurui Yang, Mohamed Hamdy Amar, Collins Ogutu, Qian Peng, Liao Liao, Jinyun Zhang, and Yuepeng Han. 2020. "Identification of EIL and ERF Genes Related to Fruit Ripening in Peach" International Journal of Molecular Sciences 21, no. 8: 2846. https://doi.org/10.3390/ijms21082846