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Article

Molecular Cytogenetic Analysis in Freshwater Prawns of the Genus Macrobrachium (Crustacea: Decapoda: Palaemonidae)

1
Departamento de Biologia Celular e Genética, Centro de Biociências, Universidade Federal do Rio Grande do Norte, Natal, RN 59078970, Brazil
2
Laboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP C.P. 676, Brazil
3
Institute for Applied Ecology, University of Canberra, Canberra, ACT 2617, Australia
4
Institute of Human Genetics, University Hospital Jena, 7747 Jena, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(7), 2599; https://doi.org/10.3390/ijms21072599
Received: 21 March 2020 / Revised: 7 April 2020 / Accepted: 7 April 2020 / Published: 9 April 2020
(This article belongs to the Special Issue Structural Variability and Flexibility of the Genome)
Freshwater prawns of the genus Macrobrachium are one of the important components of circumtropical marine, estuarine, and freshwater environments. They have been extensively exploited for human consumption for many years. More than 250 species reflect the evolutionary success of this highly diversified group, with a complex and challenging taxonomy due to morphological variations and vast geographical distribution. Although genetic approaches have been used to clarify phylogenetic and taxonomic aspects of Macrobrachium species, cytogenetic information is still very scarce and mostly focused on chromosome number and morphology. Here, we present chromosome data for three species from the Neotropical region, M. carcinus, M. acanthurus, and M. amazonicum, and one species from the Oriental region, M. rosenbergii. Using conventional cytogenetic approaches and chromosome mapping of repetitive DNAs by fluorescence in situ hybridization (FISH), we identified numerical diversification of the diploid set, within and between both zoogeographic regions. These included M. acanthurus and M. amazonicum sharing diploid chromosomes of 98, while M. carcinus has 94, and M. rosenbergii has 118 chromosomes. Argentophilic sites are also variable in number, but they occur in a much higher number than 18S rDNA, representing two to 10 sites within the study species. Microsatellites repeat motifs are also abundant in the chromosomes, with a co-localization and uniform distribution along the chromosome arms, but completely absent in the AT-rich centromeric regions. As a whole, our study suggests that the 2n divergence was followed by a considerable rDNA diversification. The abundance of the exceptional amount of microsatellite sequences in the chromosomes also suggests that they are essential components of the Macrobrachium genome and, therefore, maintained as a shared feature by the species, the reason for which is yet unknown. View Full-Text
Keywords: crustacea cytogenetics; karyotype evolution; rDNA; SSRs; diploid variation crustacea cytogenetics; karyotype evolution; rDNA; SSRs; diploid variation
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MDPI and ACS Style

Molina, W.F.; Costa, G.W.W.F.; Cunha, I.M.C.; Bertollo, L.A.C.; Ezaz, T.; Liehr, T.; Cioffi, M.B. Molecular Cytogenetic Analysis in Freshwater Prawns of the Genus Macrobrachium (Crustacea: Decapoda: Palaemonidae). Int. J. Mol. Sci. 2020, 21, 2599. https://doi.org/10.3390/ijms21072599

AMA Style

Molina WF, Costa GWWF, Cunha IMC, Bertollo LAC, Ezaz T, Liehr T, Cioffi MB. Molecular Cytogenetic Analysis in Freshwater Prawns of the Genus Macrobrachium (Crustacea: Decapoda: Palaemonidae). International Journal of Molecular Sciences. 2020; 21(7):2599. https://doi.org/10.3390/ijms21072599

Chicago/Turabian Style

Molina, Wagner F., Gideão W. W. F. Costa, Inailson M. C. Cunha, Luiz A. C. Bertollo, Tariq Ezaz, Thomas Liehr, and Marcelo B. Cioffi. 2020. "Molecular Cytogenetic Analysis in Freshwater Prawns of the Genus Macrobrachium (Crustacea: Decapoda: Palaemonidae)" International Journal of Molecular Sciences 21, no. 7: 2599. https://doi.org/10.3390/ijms21072599

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