Next Article in Journal
Amination of Graphene Oxide Leads to Increased Cytotoxicity in Hepatocellular Carcinoma Cells
Next Article in Special Issue
Stable Fibroblast Growth Factor 2 Dimers with High Pro-Survival and Mitogenic Potential
Previous Article in Journal
miR-1285-3p Controls Colorectal Cancer Proliferation and Escape from Apoptosis through DAPK2
Article

Zinc Differentially Modulates the Assembly of Soluble and Polymerized Vimentin

Department of Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, Ramiro de Maeztu, 9, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(7), 2426; https://doi.org/10.3390/ijms21072426
Received: 7 March 2020 / Revised: 27 March 2020 / Accepted: 30 March 2020 / Published: 31 March 2020
(This article belongs to the Special Issue Recent Advances in Intermediate Filaments)
The intermediate filament protein vimentin constitutes a critical sensor for electrophilic and oxidative stress. We previously showed that vimentin interacts with zinc, which affects its assembly and redox sensing. Here, we used vimentin wt and C328S, an oxidation-resistant mutant showing improved NaCl-induced polymerization, to assess the impact of zinc on soluble and polymerized vimentin by light scattering and electron microscopy. Zinc acts as a switch, reversibly inducing the formation of vimentin oligomeric species. High zinc concentrations elicit optically-detectable vimentin structures with a characteristic morphology depending on the support. These effects also occur in vimentin C328S, but are not mimicked by magnesium. Treatment of vimentin with micromolar ZnCl2 induces fibril-like particles that do not assemble into filaments, but form aggregates upon subsequent addition of NaCl. In contrast, when added to NaCl-polymerized vimentin, zinc increases the diameter or induces lateral association of vimentin wt filaments. Remarkably, these effects are absent or attenuated in vimentin C328S filaments. Therefore, the zinc-vimentin interaction depends on the chemical environment and on the assembly state of the protein, leading to atypical polymerization of soluble vimentin, likely through electrostatic interactions, or to broadening and lateral association of preformed filaments through mechanisms requiring the cysteine residue. Thus, the impact of zinc on vimentin assembly and redox regulation is envisaged. View Full-Text
Keywords: vimentin; zinc; cysteine; redox sensing; intermediate filaments; cysteine mutant; filament bundling; filament width; divalent cations; magnesium vimentin; zinc; cysteine; redox sensing; intermediate filaments; cysteine mutant; filament bundling; filament width; divalent cations; magnesium
Show Figures

Graphical abstract

MDPI and ACS Style

Mónico, A.; Zorrilla, S.; Rivas, G.; Pérez-Sala, D. Zinc Differentially Modulates the Assembly of Soluble and Polymerized Vimentin. Int. J. Mol. Sci. 2020, 21, 2426. https://doi.org/10.3390/ijms21072426

AMA Style

Mónico A, Zorrilla S, Rivas G, Pérez-Sala D. Zinc Differentially Modulates the Assembly of Soluble and Polymerized Vimentin. International Journal of Molecular Sciences. 2020; 21(7):2426. https://doi.org/10.3390/ijms21072426

Chicago/Turabian Style

Mónico, Andreia, Silvia Zorrilla, Germán Rivas, and Dolores Pérez-Sala. 2020. "Zinc Differentially Modulates the Assembly of Soluble and Polymerized Vimentin" International Journal of Molecular Sciences 21, no. 7: 2426. https://doi.org/10.3390/ijms21072426

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop