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Open AccessArticle

Lipid Deposition and Mobilisation in Atlantic Salmon Adipocytes

1
Nofima (Norwegian Institute of Food, Fisheries and Aquaculture Research), 1432 Ås, Norway
2
College of Animal Sciences, Zhejiang University, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Hangzhou 310058, China
3
Oxford Centre for Diabetes, Endocrinology and Metabolism, Radcliffe Department of Medicine, University of Oxford, Oxford OX3 7LE, UK
4
AquaGen, PO Box 1240, N-7462 Trondheim, Norway
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(7), 2332; https://doi.org/10.3390/ijms21072332
Received: 17 February 2020 / Revised: 22 March 2020 / Accepted: 25 March 2020 / Published: 27 March 2020
(This article belongs to the Special Issue Adipogenesis and Adipose Tissue Metabolism)
The present study aimed to elucidate how Atlantic salmon adipocytes pre-enriched with palmitic (16:0, PA), oleic (18:1n−9, OA), or eicosapentaenoic (20:5n−3, EPA) acid respond to a fasting condition mimicked by nutrient deprivation and glucagon. All experimental groups were supplemented with radiolabeled PA to trace secreted lipids and distribution of radioactivity in different lipid classes. There was a higher content of intracellular lipid droplets in adipocytes pre-enriched with OA than in adipocytes pre-enriched with PA or EPA. In the EPA group, the radiolabeled PA was mainly esterified in phospholipids and triacylglycerols, whereas in the OA and PA groups, the radioactivity was mainly recovered in phospholipids and cholesterol-ester. By subjecting the experimental groups to nutrient-deprived media supplemented with glucagon, lipolysis occurred in all groups, although to a lower extent in the OA group. The lipids were mainly secreted as esterified lipids in triacylglycerols and phospholipids, indicating mobilization in lipoproteins. A significant proportion was secreted as free fatty acids and glycerol. Leptin secretion was reduced in all experimental groups in response to fasting, while the mitochondria area responded to changes in the energy supply and demand by increasing after 3 h of fasting. Overall, different lipid classes in adipocytes influenced their mobilization during fasting. View Full-Text
Keywords: lipogenesis; lipolysis; fasting; leptin; mitochondria; Salmo salar lipogenesis; lipolysis; fasting; leptin; mitochondria; Salmo salar
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MDPI and ACS Style

Bou, M.; Wang, X.; Todorčević, M.; Østbye, T.-K.K.; Torgersen, J.; Ruyter, B. Lipid Deposition and Mobilisation in Atlantic Salmon Adipocytes. Int. J. Mol. Sci. 2020, 21, 2332. https://doi.org/10.3390/ijms21072332

AMA Style

Bou M, Wang X, Todorčević M, Østbye T-KK, Torgersen J, Ruyter B. Lipid Deposition and Mobilisation in Atlantic Salmon Adipocytes. International Journal of Molecular Sciences. 2020; 21(7):2332. https://doi.org/10.3390/ijms21072332

Chicago/Turabian Style

Bou, Marta; Wang, Xinxia; Todorčević, Marijana; Østbye, Tone-Kari K.; Torgersen, Jacob; Ruyter, Bente. 2020. "Lipid Deposition and Mobilisation in Atlantic Salmon Adipocytes" Int. J. Mol. Sci. 21, no. 7: 2332. https://doi.org/10.3390/ijms21072332

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