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Open AccessArticle

Analysis of the Activity and Expression of Cyclooxygenases COX1 and COX2 in THP-1 Monocytes and Macrophages Cultured with BiodentineTM Silicate Cement

1
Department of Conservative Dentistry and Endodontics, Pomeranian Medical University, Powstańców Wlkp 72, 70-111 Szczecin, Poland
2
Department of Biochemistry and Medical Chemistry, Pomeranian Medical University, Powstańców Wlkp, 70-111 Szczecin, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(6), 2237; https://doi.org/10.3390/ijms21062237
Received: 12 March 2020 / Accepted: 20 March 2020 / Published: 24 March 2020
(This article belongs to the Section Materials Science)
BiodentineTM is a material based on hydrated calcium silicate with odontotropic properties. However, from the clinician’s perspective, every material used to fill a tooth—even those showing the optimal biochemical parameters—is in fact a foreign body introduced to the organism of the host. Therefore, apart from the chemical parameters of such materials, equally important is the so-called biocompatibility of such materials. The aim of the study was to investigate whether BiodentineTM, used in the regeneration of the pulp-dentine complex, may affect the expression of the enzymes cyclooxygenase 1 (COX1) and cyclooxygenase 2 (COX2) in THP-1 monocytes/macrophages and the amount of prostanoids synthesized by these enzymes-precursors of biologically active prostanoids such as prostaglandin E2 (PGE2) and thromboxane (TXB2) which are mediators of inflammation. An original aspect of this research is the use of the THP-1 monocyte/macrophage cell model and the use of biomaterial in direct contact with cells. In this way we tried to reflect the clinical conditions of regenerative pulp and periodontal tissue treatment using BiodentineTM. The results of our study showed a lack of macrophage activation (measured by flow cytometry) and a lack of stimulation of the expression of the studied cyclooxygenase enzymes (measured by Western blotting and fluorescent microscopy), as well as a lack of increase in the concentration (measured by ELISA method) of their inflammatory mediators (PGE2 and TXB2) in vitro incubated with BiodentineTM. View Full-Text
Keywords: BiodentineTM; bioactive calcium-silicate cement; THP-1 monocytes; macrophages; cyclooxygenase-1 (COX-1); cyclooxygenase-2 (COX-2); inflammatory reaction BiodentineTM; bioactive calcium-silicate cement; THP-1 monocytes; macrophages; cyclooxygenase-1 (COX-1); cyclooxygenase-2 (COX-2); inflammatory reaction
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MDPI and ACS Style

Barczak, K.; Palczewska-Komsa, M.; Nowicka, A.; Chlubek, D.; Buczkowska-Radlińska, J. Analysis of the Activity and Expression of Cyclooxygenases COX1 and COX2 in THP-1 Monocytes and Macrophages Cultured with BiodentineTM Silicate Cement. Int. J. Mol. Sci. 2020, 21, 2237.

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