The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion
Abstract
:1. Introduction
2. Results
2.1. Effect of Optogenetic Activation of DRN 5-HT Neurons Transduced by AAV Bearing Mouse TPH2 Promoter in Nose-Poke Self-Stimulation Test and Conditioned Place Preference (CPP) Test
2.2. Optogenetic Activation of 5-HT Neuron Terminals in the VTA is Responsible for Reinforcement of Nose-Poke Behavior but not in the LH, CeA, NAc, or Ventral Pallidum (VP)
2.3. Effect of Optogenetic Activation of DRN 5-HT Neurons Targeted by Tph2-tTA Transgenic Mice in Nose-Poke Self-Stimulation Test and CPP Test
2.4. Optogenetic Inhibition of DRN 5-HT Neurons and Their Terminals in the VTA Elicits Conditioned Place Aversion
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Stereotaxic Surgeries
4.3. Vector Construction
4.4. Production and Purification of Adeno-Associated Virus (AAV) Vector
4.5. In Vivo Optogenetic Manipulation
4.6. Behavioral Tests
4.7. Self-Stimulation in an Operant Chamber
4.8. Conditioned Place Preference (CPP) Test
4.9. Conditioned Place Aversion (CPA) Test
4.10. Histology
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Nagai, Y.; Takayama, K.; Nishitani, N.; Andoh, C.; Koda, M.; Shirakawa, H.; Nakagawa, T.; Nagayasu, K.; Yamanaka, A.; Kaneko, S. The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion. Int. J. Mol. Sci. 2020, 21, 2160. https://doi.org/10.3390/ijms21062160
Nagai Y, Takayama K, Nishitani N, Andoh C, Koda M, Shirakawa H, Nakagawa T, Nagayasu K, Yamanaka A, Kaneko S. The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion. International Journal of Molecular Sciences. 2020; 21(6):2160. https://doi.org/10.3390/ijms21062160
Chicago/Turabian StyleNagai, Yuma, Kaito Takayama, Naoya Nishitani, Chihiro Andoh, Masashi Koda, Hisashi Shirakawa, Takayuki Nakagawa, Kazuki Nagayasu, Akihiro Yamanaka, and Shuji Kaneko. 2020. "The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion" International Journal of Molecular Sciences 21, no. 6: 2160. https://doi.org/10.3390/ijms21062160