Genome-Wide Analysis of the Shi-Related Sequence Family and Functional Identification of GmSRS18 Involving in Drought and Salt Stresses in Soybean
Abstract
:1. Introduction
2. Results
2.1. Identification of SRS Transcription Factors in Soybean
2.2. Analysis of Gene Structure and Cis-Acting Elements
2.3. Analysis of Expression Patterns in Various Tissues
2.4. RNA-Seq Analysis of Drought-, NaCl-, and ABA-responsive SRS Genes
2.5. Quantitative Real-Time PCR (qRT-PCR) Analysis of Soybean SRS Genes
2.6. Subcellular Localization of GmSRS18 in Arabidopsis Protoplasts
2.7. Overexpression of GmSRS18 Conferred Drought and Salt sensitivity in Arabidopsis
2.8. GmSRS18 Negatively Regulated Drought- and Salt-Related Gene Expression
3. Discussion
4. Materials and Methods
4.1. The Search of All Members in SRS Family in the Soybean and Arabidopsis
4.2. Phylogenetic Analysis and Alignment of SRSs Sequences
4.3. Chromosome Locations of Soybean SRS Genes
4.4. Gene Structure and cis-acting Element Analysis
4.5. The Expression Patterns Analysis of SRS Genes in Different Tissues
4.6. Plant Materials and Stress Treatments
4.7. Soybean RNA-seq and Quantitative Real-time PCR (qRT-PCR)
4.8. Subcellular Localization Analysis of GmSRS18
4.9. Functional analysis of GmSRS18 in Arabidopsis
4.10. Physiological Measurements
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ABA | abscisic acid |
qRT-PCR | quantitative Real-Time PCR |
WT | wild type |
ABRE | ABA-responsive element |
GFP | Green fluorescent protein |
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Gene Name | Gene ID | Amino Acid Residues (aa) | Molecular Weight (MW) (Da) | PI | Chromosome | RING-LIKE Zinc Finger Domain | Bes Hit in Arabidopsis |
---|---|---|---|---|---|---|---|
GmSRS1 | Glyma.01G170700 | 317 | 34290.8 | 6.5009 | 1 | 103~256 | STY1 |
GmSRS2 | Glyma.02G051900 | 329 | 35460.7 | 7.3971 | 2 | 115~259 | STY1 |
GmSRS3 | Glyma.02G280000 | 356 | 37500.2 | 8.3444 | 2 | 135~296 | LRP1 |
GmSRS4 | Glyma.04G009300 | 211 | 23087.5 | 7.9448 | 4 | 12~62 | SRS3 |
GmSRS5 | Glyma.04G027400 | 306 | 33185.7 | 6.8469 | 4 | 98~245 | SRS5 |
GmSRS6 | Glyma.04G136700 | 333 | 35377.4 | 8.1854 | 4 | 135~287 | LRP1 |
GmSRS7 | Glyma.06G009200 | 201 | 22323.7 | 8.4033 | 6 | 21~77 | SRS3 |
GmSRS8 | Glyma.06G027500 | 302 | 33032.5 | 7.7425 | 6 | 103~242 | SRS7 |
GmSRS9 | Glyma.07G230400 | 331 | 35295.6 | 7.0258 | 7 | 117~277 | LRP1 |
GmSRS10 | Glyma.11G072500 | 335 | 36133.9 | 6.6899 | 11 | 111~269 | SRS5 |
GmSRS11 | Glyma.11G113200 | 216 | 24179.3 | 9.1277 | 11 | 7~70 | SRS3 |
GmSRS12 | Glyma.11G155400 | 332 | 35606.7 | 8.5013 | 11 | 137~285 | LRP1 |
GmSRS13 | Glyma.12G039100 | 211 | 23370.3 | 8.5773 | 12 | 10~138 | SRS3 |
GmSRS14 | Glyma.13G197300 | 320 | 34173.9 | 8.0081 | 13 | 122~278 | LRP1 |
GmSRS15 | Glyma.14G034800 | 350 | 36900.8 | 8.3423 | 14 | 133~293 | LRP1 |
GmSRS16 | Glyma.14G216400 | 334 | 36073.6 | 7.2692 | 14 | 117~268 | SRS5 |
GmSRS17 | Glyma.15G235000 | 323 | 35013.6 | 7.7249 | 15 | 126~281 | LRP1 |
GmSRS18 | Glyma.16G132100 | 315 | 34541.7 | 7.1072 | 16 | 109~247 | SRS7 |
GmSRS19 | Glyma.17G150800 | 371 | 41022.1 | 5.765 | 17 | 45~81 | SRS11 |
GmSRS20 | Glyma.17G255000 | 327 | 35594.1 | 7.2575 | 17 | 111~262 | SRS5 |
GmSRS21 | Glyma.20G037100 | 341 | 36080.5 | 7.5825 | 20 | 113~274 | LRP1 |
Gene | ABRE | E-BOX | GT-1 | LTRE | MYB | MYC |
---|---|---|---|---|---|---|
GmSRS1 | 10 | 8 | 16 | 2 | 4 | 10 |
GmSRS2 | 8 | 14 | 25 | 2 | 12 | 16 |
GmSRS3 | 2 | 10 | 35 | 0 | 3 | 14 |
GmSRS4 | 3 | 10 | 30 | 1 | 6 | 12 |
GmSRS5 | 6 | 16 | 27 | 1 | 8 | 18 |
GmSRS6 | 3 | 14 | 35 | 3 | 3 | 18 |
GmSRS7 | 2 | 18 | 39 | 1 | 2 | 22 |
GmSRS8 | 9 | 14 | 24 | 0 | 8 | 16 |
GmSRS9 | 6 | 18 | 16 | 1 | 7 | 26 |
GmSRS10 | 8 | 8 | 26 | 3 | 1 | 30 |
GmSRS11 | 1 | 8 | 25 | 0 | 4 | 14 |
GmSRS12 | 3 | 9 | 51 | 0 | 4 | 8 |
GmSRS13 | 5 | 14 | 36 | 0 | 6 | 14 |
GmSRS14 | 2 | 12 | 51 | 1 | 3 | 12 |
GmSRS15 | 2 | 14 | 35 | 1 | 5 | 18 |
GmSRS16 | 9 | 20 | 31 | 1 | 4 | 22 |
GmSRS17 | 5 | 8 | 35 | 0 | 4 | 8 |
GmSRS18 | 8 | 8 | 26 | 0 | 14 | 10 |
GmSRS19 | 4 | 8 | 27 | 0 | 4 | 8 |
GmSRS20 | 7 | 18 | 27 | 1 | 6 | 20 |
GmSRS21 | 1 | 10 | 37 | 1 | 4 | 16 |
Total | 104 | 259 | 654 | 19 | 112 | 332 |
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Zhao, S.-P.; Song, X.-Y.; Guo, L.-L.; Zhang, X.-Z.; Zheng, W.-J. Genome-Wide Analysis of the Shi-Related Sequence Family and Functional Identification of GmSRS18 Involving in Drought and Salt Stresses in Soybean. Int. J. Mol. Sci. 2020, 21, 1810. https://doi.org/10.3390/ijms21051810
Zhao S-P, Song X-Y, Guo L-L, Zhang X-Z, Zheng W-J. Genome-Wide Analysis of the Shi-Related Sequence Family and Functional Identification of GmSRS18 Involving in Drought and Salt Stresses in Soybean. International Journal of Molecular Sciences. 2020; 21(5):1810. https://doi.org/10.3390/ijms21051810
Chicago/Turabian StyleZhao, Shu-Ping, Xin-Yuan Song, Lin-Lin Guo, Xiang-Zhan Zhang, and Wei-Jun Zheng. 2020. "Genome-Wide Analysis of the Shi-Related Sequence Family and Functional Identification of GmSRS18 Involving in Drought and Salt Stresses in Soybean" International Journal of Molecular Sciences 21, no. 5: 1810. https://doi.org/10.3390/ijms21051810