Understanding Mechanisms of Salinity Tolerance in Barley by Proteomic and Biochemical Analysis of Near-Isogenic Lines
Abstract
:1. Introduction
2. Results
2.1. Near Isogenic Lines Exhibited Contrasting Salinity Tolerance
2.2. Effect of Salinity on Na+ and K+ Contents and Na+/ K+ Ratios
2.3. The Accumulation of H2O2, MDA, and Proline After Salinity Treatment
2.4. Changes in Activities of Antioxidant (AO) Enzymes under Salinity Stress
2.5. Identification of Differentially Expressed Proteins
3. Discussion
3.1. Near Isogenic Lines With Contrasting Salinity Tolerance Poses Less Background Noise
3.2. The Effects of QSl.TxNn.2H on Biochemical Characteristics and Plant Ionome
3.3. ROS Scavenging, Photosynthesis, and ATP Synthase Related Proteins Upregulated in Salinity Tolerant Lines and Underly the Tolerance Mechanism
4. Materials and Methods
4.1. DNA Extraction and Genotype Screening
4.2. Development of Near Isogenic Lines (NILs)
4.3. Growth Condition and Materials Collection
4.4. Determination of H2O2, MDA and Proline Measurements Contents
4.5. Antioxidant Enzyme Activity Measurements
4.6. Na+ and K+ Content
4.7. Protein Extraction, 2-DE, and Image Analysis
4.8. Identification of Proteins by Mass Spectrometry (MS)
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Zhu, J.; Fan, Y.; Shabala, S.; Li, C.; Lv, C.; Guo, B.; Xu, R.; Zhou, M. Understanding Mechanisms of Salinity Tolerance in Barley by Proteomic and Biochemical Analysis of Near-Isogenic Lines. Int. J. Mol. Sci. 2020, 21, 1516. https://doi.org/10.3390/ijms21041516
Zhu J, Fan Y, Shabala S, Li C, Lv C, Guo B, Xu R, Zhou M. Understanding Mechanisms of Salinity Tolerance in Barley by Proteomic and Biochemical Analysis of Near-Isogenic Lines. International Journal of Molecular Sciences. 2020; 21(4):1516. https://doi.org/10.3390/ijms21041516
Chicago/Turabian StyleZhu, Juan, Yun Fan, Sergey Shabala, Chengdao Li, Chao Lv, Baojian Guo, Rugen Xu, and Meixue Zhou. 2020. "Understanding Mechanisms of Salinity Tolerance in Barley by Proteomic and Biochemical Analysis of Near-Isogenic Lines" International Journal of Molecular Sciences 21, no. 4: 1516. https://doi.org/10.3390/ijms21041516