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Article

Comparative Proteomic Analysis of Wild-Type Physcomitrella Patens and an OPDA-Deficient Physcomitrella Patens Mutant with Disrupted PpAOS1 and PpAOS2 Genes after Wounding

1
Division of Fundamental Agroscience Research, Research Faculty of Agriculture, Hokkaido University, Kita 9, Nishi 9, Kita-ku, Sapporo 060-8589, Japan
2
Department of Environmental and Food Sciences, Faculty of Environmental and Information Sciences, Fukui University of Technology, 3-6-1 Gakuen, Fukui 910-8505, Japan
3
Department of Nutritional Science, Faculty of Applied Bioscience, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 165-8502, Japan
*
Author to whom correspondence should be addressed.
Present Address: Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba 305-8602, Japan.
Present Address: Research Center, Nippon Beet Sugar MFG. Co., Ltd. Inada-cho, Obihiro, Hokkaido 080-8031, Japan.
Int. J. Mol. Sci. 2020, 21(4), 1417; https://doi.org/10.3390/ijms21041417
Received: 17 January 2020 / Revised: 13 February 2020 / Accepted: 17 February 2020 / Published: 19 February 2020
(This article belongs to the Special Issue Plant Proteomic Research 3.0)
Wounding is a serious environmental stress in plants. Oxylipins such as jasmonic acid play an important role in defense against wounding. Mechanisms to adapt to wounding have been investigated in vascular plants; however, those mechanisms in nonvascular plants remain elusive. To examine the response to wounding in Physcomitrella patens, a model moss, a proteomic analysis of wounded P. patens was conducted. Proteomic analysis showed that wounding increased the abundance of proteins related to protein synthesis, amino acid metabolism, protein folding, photosystem, glycolysis, and energy synthesis. 12-Oxo-phytodienoic acid (OPDA) was induced by wounding and inhibited growth. Therefore, OPDA is considered a signaling molecule in this plant. Proteomic analysis of a P. patens mutant in which the PpAOS1 and PpAOS2 genes, which are involved in OPDA biosynthesis, are disrupted showed accumulation of proteins involved in protein synthesis in response to wounding in a similar way to the wild-type plant. In contrast, the fold-changes of the proteins in the wild-type plant were significantly different from those in the aos mutant. This study suggests that PpAOS gene expression enhances photosynthesis and effective energy utilization in response to wounding in P. patens. View Full-Text
Keywords: Allene oxide synthase; 12-oxo-phytodienoic acid; Physcomitrella patens; proteomic analysis; wounding Allene oxide synthase; 12-oxo-phytodienoic acid; Physcomitrella patens; proteomic analysis; wounding
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MDPI and ACS Style

Luo, W.; Komatsu, S.; Abe, T.; Matsuura, H.; Takahashi, K. Comparative Proteomic Analysis of Wild-Type Physcomitrella Patens and an OPDA-Deficient Physcomitrella Patens Mutant with Disrupted PpAOS1 and PpAOS2 Genes after Wounding. Int. J. Mol. Sci. 2020, 21, 1417. https://doi.org/10.3390/ijms21041417

AMA Style

Luo W, Komatsu S, Abe T, Matsuura H, Takahashi K. Comparative Proteomic Analysis of Wild-Type Physcomitrella Patens and an OPDA-Deficient Physcomitrella Patens Mutant with Disrupted PpAOS1 and PpAOS2 Genes after Wounding. International Journal of Molecular Sciences. 2020; 21(4):1417. https://doi.org/10.3390/ijms21041417

Chicago/Turabian Style

Luo, Weifeng, Setsuko Komatsu, Tatsuya Abe, Hideyuki Matsuura, and Kosaku Takahashi. 2020. "Comparative Proteomic Analysis of Wild-Type Physcomitrella Patens and an OPDA-Deficient Physcomitrella Patens Mutant with Disrupted PpAOS1 and PpAOS2 Genes after Wounding" International Journal of Molecular Sciences 21, no. 4: 1417. https://doi.org/10.3390/ijms21041417

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