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Article

Iron Overload Mimicking Conditions Skews Bone Marrow Dendritic Cells Differentiation into MHCIIlowCD11c+CD11b+F4/80+ Cells

1
National Institute of Gastroenterology “S. de Bellis”, Research Hospital, Castellana Grotte, 70013 Bari, Italy
2
Department of Immunology and Cell Biology, European Biomedical Research Institute of Salerno (EBRIS), 84125 Salerno, Italy
3
Department of Pharmacy, University of Salerno, 84084 Fisciano, Italy
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Department of Pharmacy-Drug Science, University of Bari Aldo Moro, 70126 Bari, Italy
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Unit of Lecce, Institute of Sciences of Food Production C.N.R., via Monteroni, 73100 Lecce, Italy
6
Harvard Medical School Division of Pediatric Gastroenterology and Nutrition and Mucosal Immunology and Biology Research Center, Massachusetts General Hospital for Children, Boston, MA 02114, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(4), 1353; https://doi.org/10.3390/ijms21041353
Received: 29 January 2020 / Revised: 14 February 2020 / Accepted: 15 February 2020 / Published: 17 February 2020
(This article belongs to the Special Issue Role of Dendritic Cells in Inflammation)
Iron overload is an undesired effect of frequent blood transfusions or genetic diseases. Myelodysplastic syndrome (MDS) patients become transfusion dependent, but due to the combination of ineffective haematopoiesis and repeated blood transfusions they are often subject to iron overload. In this study, we demonstrate that iron-overload mimicking condition alters bone marrow progenitor differentiation towards dendritic cells (DCs). Cells cultured in iron-enriched culture medium for seven days fail to differentiate into conventional CD11c+MHCIIhi DCs and fail to efficiently respond to LPS (Lipopolysaccharides). Cells appear smaller than control DCs but vital and able to perform FITC-dextran (Fluorescein isothiocyanate-dextran) endocytosis. At molecular level, cells cultured in iron-enriched conditions show increased ARG1 and PU.1, and decreased IRF8 expression. View Full-Text
Keywords: dendritic cells; inflammation; iron overload; bone marrow dendritic cells; inflammation; iron overload; bone marrow
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MDPI and ACS Style

Verna, G.; Liso, M.; De Santis, S.; Dicarlo, M.; Cavalcanti, E.; Crovace, A.; Sila, A.; Campiglia, P.; Santino, A.; Lippolis, A.; Serino, G.; Fasano, A.; Chieppa, M. Iron Overload Mimicking Conditions Skews Bone Marrow Dendritic Cells Differentiation into MHCIIlowCD11c+CD11b+F4/80+ Cells. Int. J. Mol. Sci. 2020, 21, 1353. https://doi.org/10.3390/ijms21041353

AMA Style

Verna G, Liso M, De Santis S, Dicarlo M, Cavalcanti E, Crovace A, Sila A, Campiglia P, Santino A, Lippolis A, Serino G, Fasano A, Chieppa M. Iron Overload Mimicking Conditions Skews Bone Marrow Dendritic Cells Differentiation into MHCIIlowCD11c+CD11b+F4/80+ Cells. International Journal of Molecular Sciences. 2020; 21(4):1353. https://doi.org/10.3390/ijms21041353

Chicago/Turabian Style

Verna, Giulio, Marina Liso, Stefania De Santis, Manuela Dicarlo, Elisabetta Cavalcanti, Alberto Crovace, Annamaria Sila, Pietro Campiglia, Angelo Santino, Antonio Lippolis, Grazia Serino, Alessio Fasano, and Marcello Chieppa. 2020. "Iron Overload Mimicking Conditions Skews Bone Marrow Dendritic Cells Differentiation into MHCIIlowCD11c+CD11b+F4/80+ Cells" International Journal of Molecular Sciences 21, no. 4: 1353. https://doi.org/10.3390/ijms21041353

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