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Genetic Variants Detected Using Cell-Free DNA from Blood and Tumor Samples in Patients with Inflammatory Breast Cancer

Fox Chase Cancer Center, Philadelphia, PA 19111, USA
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Author to whom correspondence should be addressed.
Present address: Department of Medicine-Hematology and Oncology, Robert H Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
Int. J. Mol. Sci. 2020, 21(4), 1290; https://doi.org/10.3390/ijms21041290
Received: 9 January 2020 / Revised: 11 February 2020 / Accepted: 12 February 2020 / Published: 14 February 2020
(This article belongs to the Section Molecular Oncology)
We studied genomic alterations in 19 inflammatory breast cancer (IBC) patients with advanced disease using samples of tissue and paired blood serum or plasma (cell-free DNA, cfDNA) by targeted next generation sequencing (NGS). At diagnosis, the disease was triple negative (TN) in eleven patients (57.8%), ER+ Her2- IBC in six patients (31.6%), ER+ Her2+ IBC in one patient (5.3%), and ER- Her2+ IBC in one other patient (5.3%). Pathogenic or likely pathogenic variants were frequently detected in TP53 (47.3%), PMS2 (26.3%), MRE11 (26.3%), RB1 (10.5%), BRCA1 (10.5%), PTEN (10.5%) and AR (10.5%); other affected genes included PMS1, KMT2C, BRCA2, PALB2, MUTYH, MEN1, MSH2, CHEK2, NCOR1, PIK3CA, ESR1 and MAP2K4. In 15 of the 19 patients in which tissue and paired blood were collected at the same time point, 80% of the variants detected in tissue were also detected in the paired cfDNA. Higher concordance between tissue and cfDNA was found for variants with higher allele fraction in tissue (AFtissue ≥ 5%). Furthermore, 86% of the variants detected in cfDNA were also detected in paired tissue. Our study suggests that the genetic profile measured in blood cfDNA is complementary to that of tumor tissue in IBC patients. View Full-Text
Keywords: inflammatory breast cancer (IBC); cell-free DNA (cfDNA); next generation sequencing (NGS) inflammatory breast cancer (IBC); cell-free DNA (cfDNA); next generation sequencing (NGS)
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Winn, J.S.; Hasse, Z.; Slifker, M.; Pei, J.; Arisi-Fernandez, S.M.; Talarchek, J.N.; Obeid, E.; Baldwin, D.A.; Gong, Y.; Ross, E.; Cristofanilli, M.; Alpaugh, R.K.; Fernandez, S.V. Genetic Variants Detected Using Cell-Free DNA from Blood and Tumor Samples in Patients with Inflammatory Breast Cancer. Int. J. Mol. Sci. 2020, 21, 1290.

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