Potential Molecular Mimicry Proteins Responsive to α-pinene in Bursaphelenchus xylophilus
Abstract
:1. Introduction
2. Results
2.1. Cloning of Bx-tlp-2 and Bx-cpi
2.2. Analysis of the Bx-TLP-2 and Bx-CPI Proteins
2.3. Genetic Relationship between Bx-tlp-2 and Bx-cpi
2.4. Expression of Bx-tlp-1, Bx-tlp-2, Bx-cpi and Bx-cpl-1
2.5. Expression Patterns of Potential Molecular Mimicry Proteins from B. xylophilus and P. massoniana
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. RNA Isolation and cDNA Cloning
4.3. Verification of the Relationship between α-pinene and the Potential Molecular Mimicry Proteins in Bursaphelenchus xylophilus
4.4. Expression Analysis of the Potential Molecular Mimicry Proteins from Bursaphelenchus xylophilus and Pinus massoniana
4.5. Data Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Amino acids | Number | Percentage | Amino Acids | Number | Percentage | Amino Acids | Number | Percentage |
---|---|---|---|---|---|---|---|---|
Ala (A) | 4 | 4.7% | Gly (G) | 11 | 12.9% | Phe (F) | 5 | 5.9% |
Arg (R) | 4 | 4.7% | His (H) | 1 | 1.2% | Pro (P) | 2 | 2.4% |
Asn (N) | 4 | 4.7% | Ile (I) | 1 | 1.2% | Ser (S) | 6 | 7.1% |
Asp (D) | 8 | 9.4% | Leu (L) | 9 | 10.6% | Thr (T) | 7 | 8.2% |
Gln (Q) | 1 | 1.2% | Lys (K) | 1 | 1.2% | Tyr (Y) | 7 | 8.2% |
Glu (Q) | 4 | 4.7% | Met (M) | 2 | 2.4% | Val (V) | 4 | 4.7% |
Cys (C) | 4 | 4.7% |
Amino acids | Number | Percentage | Amino Acids | Number | Percentage | Amino Acids | Number | Percentage |
---|---|---|---|---|---|---|---|---|
Ala (A) | 10 | 8.1% | Gly (G) | 4 | 3.2% | Phe (F) | 7 | 5.6% |
Arg (R) | 4 | 3.2% | His (H) | 7 | 5.6% | Pro (P) | 5 | 4.0% |
Asn (N) | 2 | 1.6% | Ile (I) | 5 | 4.0% | Ser (S) | 4 | 3.2% |
Asp (D) | 3 | 2.4% | Leu (L) | 13 | 10.5% | Thr (T) | 3 | 2.4% |
Gln (Q) | 14 | 11.3% | Lys (K) | 18 | 14.5% | Val (V) | 22 | 17.7% |
Glu (Q) | 2 | 1.6% | Met (M) | 1 | 0.8% |
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Meng, F.; Li, Y.; Liu, Z.; Wang, X.; Feng, Y.; Zhang, W.; Zhang, X. Potential Molecular Mimicry Proteins Responsive to α-pinene in Bursaphelenchus xylophilus. Int. J. Mol. Sci. 2020, 21, 982. https://doi.org/10.3390/ijms21030982
Meng F, Li Y, Liu Z, Wang X, Feng Y, Zhang W, Zhang X. Potential Molecular Mimicry Proteins Responsive to α-pinene in Bursaphelenchus xylophilus. International Journal of Molecular Sciences. 2020; 21(3):982. https://doi.org/10.3390/ijms21030982
Chicago/Turabian StyleMeng, Fanli, Yongxia Li, Zhenkai Liu, Xuan Wang, Yuqian Feng, Wei Zhang, and Xingyao Zhang. 2020. "Potential Molecular Mimicry Proteins Responsive to α-pinene in Bursaphelenchus xylophilus" International Journal of Molecular Sciences 21, no. 3: 982. https://doi.org/10.3390/ijms21030982