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Transcriptome Analysis of Rice Roots in Response to Root-Knot Nematode Infection

College of Plant Protection, Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, China
Shaanxi Key Laboratory of Chinese Jujube, College of Life Science, Yan’an University, Yan’an, Shaanxi 716000, China
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(3), 848;
Received: 6 December 2019 / Revised: 24 January 2020 / Accepted: 27 January 2020 / Published: 28 January 2020
(This article belongs to the Special Issue Plant Disease Resistance)
Meloidogyne incognita and Meloidogyne graminicola are root-knot nematodes (RKNs) infecting rice (Oryza sativa L.) roots and severely decreasing yield, whose mechanisms of action remain unclear. We investigated RKN invasion and development in rice roots through RNA-seq transcriptome analysis. The results showed that 952 and 647 genes were differently expressed after 6 (invasion stage) and 18 (development stage) days post inoculation, respectively. Gene annotation showed that the differentially expressed genes were classified into diverse metabolic and stress response categories. Furthermore, phytohormone, transcription factor, redox signaling, and defense response pathways were enriched upon RKN infection. RNA-seq validation using qRT-PCR confirmed that CBL-interacting protein kinase (CIPK) genes (CIPK5, 8, 9, 11, 14, 23, 24, and 31) as well as brassinosteroid (BR)-related genes (OsBAK1, OsBRI1, D2, and D11) were altered by RKN infection. Analysis of the CIPK9 mutant and overexpressor indicated that the RKN populations were smaller in cipk9 and larger in CIPK9 OX, while more galls were produced in CIPK9 OX plant roots than the in wild-type roots. Significantly fewer numbers of second-stage infective juveniles (J2s) were observed in the plants expressing the BR biosynthesis gene D2 mutant and the BR receptor BRI1 activation-tagged mutant (bri1-D), and fewer galls were observed in bri1-D roots than in wild-type roots. The roots of plants expressing the regulator of ethylene signaling ERS1 (ethylene response sensor 1) mutant contained higher numbers of J2s and developed more galls compared with wild-type roots, suggesting that these signals function in RKN invasion or development. Our findings broaden our understanding of rice responses to RKN invasion and provide useful information for further research on RKN defense mechanisms. View Full-Text
Keywords: root-knot nematode; plant defense; rice; Meloidogyne incognita root-knot nematode; plant defense; rice; Meloidogyne incognita
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MDPI and ACS Style

Zhou, Y.; Zhao, D.; Shuang, L.; Xiao, D.; Xuan, Y.; Duan, Y.; Chen, L.; Wang, Y.; Liu, X.; Fan, H.; Zhu, X. Transcriptome Analysis of Rice Roots in Response to Root-Knot Nematode Infection. Int. J. Mol. Sci. 2020, 21, 848.

AMA Style

Zhou Y, Zhao D, Shuang L, Xiao D, Xuan Y, Duan Y, Chen L, Wang Y, Liu X, Fan H, Zhu X. Transcriptome Analysis of Rice Roots in Response to Root-Knot Nematode Infection. International Journal of Molecular Sciences. 2020; 21(3):848.

Chicago/Turabian Style

Zhou, Yuan, Di Zhao, Li Shuang, Dongxue Xiao, Yuanhu Xuan, Yuxi Duan, Lijie Chen, Yuanyuan Wang, Xiaoyu Liu, Haiyan Fan, and Xiaofeng Zhu. 2020. "Transcriptome Analysis of Rice Roots in Response to Root-Knot Nematode Infection" International Journal of Molecular Sciences 21, no. 3: 848.

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