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Transcriptome Analysis of Drought-Resistant and Drought-Sensitive Sorghum (Sorghum bicolor) Genotypes in Response to PEG-Induced Drought Stress

1
Department of Biology and Cell and Molecular Biology Program, Colorado State University, Fort Collins, CO 80523, USA
2
Computer Science Department, Colorado State University, Fort Collins, CO 80523, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(3), 772; https://doi.org/10.3390/ijms21030772
Received: 28 December 2019 / Revised: 20 January 2020 / Accepted: 22 January 2020 / Published: 24 January 2020
(This article belongs to the Section Molecular Plant Sciences)
Drought is a major limiting factor of crop yields. In response to drought, plants reprogram their gene expression, which ultimately regulates a multitude of biochemical and physiological processes. The timing of this reprogramming and the nature of the drought-regulated genes in different genotypes are thought to confer differential tolerance to drought stress. Sorghum is a highly drought-tolerant crop and has been increasingly used as a model cereal to identify genes that confer tolerance. Also, there is considerable natural variation in resistance to drought in different sorghum genotypes. Here, we evaluated drought resistance in four genotypes to polyethylene glycol (PEG)-induced drought stress at the seedling stage and performed transcriptome analysis in seedlings of sorghum genotypes that are either drought-resistant or drought-sensitive to identify drought-regulated changes in gene expression that are unique to drought-resistant genotypes of sorghum. Our analysis revealed that about 180 genes are differentially regulated in response to drought stress only in drought-resistant genotypes and most of these (over 70%) are up-regulated in response to drought. Among these, about 70 genes are novel with no known function and the remaining are transcription factors, signaling and stress-related proteins implicated in drought tolerance in other crops. This study revealed a set of drought-regulated genes, including many genes encoding uncharacterized proteins that are associated with drought tolerance at the seedling stage. View Full-Text
Keywords: sorghum; transcriptome; polyethylene glycol (PEG); drought; gene expression; drought resistance sorghum; transcriptome; polyethylene glycol (PEG); drought; gene expression; drought resistance
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MDPI and ACS Style

Abdel-Ghany, S.E.; Ullah, F.; Ben-Hur, A.; Reddy, A.S.N. Transcriptome Analysis of Drought-Resistant and Drought-Sensitive Sorghum (Sorghum bicolor) Genotypes in Response to PEG-Induced Drought Stress. Int. J. Mol. Sci. 2020, 21, 772. https://doi.org/10.3390/ijms21030772

AMA Style

Abdel-Ghany SE, Ullah F, Ben-Hur A, Reddy ASN. Transcriptome Analysis of Drought-Resistant and Drought-Sensitive Sorghum (Sorghum bicolor) Genotypes in Response to PEG-Induced Drought Stress. International Journal of Molecular Sciences. 2020; 21(3):772. https://doi.org/10.3390/ijms21030772

Chicago/Turabian Style

Abdel-Ghany, Salah E., Fahad Ullah, Asa Ben-Hur, and Anireddy S.N. Reddy 2020. "Transcriptome Analysis of Drought-Resistant and Drought-Sensitive Sorghum (Sorghum bicolor) Genotypes in Response to PEG-Induced Drought Stress" International Journal of Molecular Sciences 21, no. 3: 772. https://doi.org/10.3390/ijms21030772

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