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Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37

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Department of Physiology and Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia
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Division of Molecular Medicine, Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia
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Department of Medical Biology and Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, 03601 Martin, Slovakia
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Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 1148; https://doi.org/10.3390/ijms21031148
Received: 13 January 2020 / Revised: 2 February 2020 / Accepted: 7 February 2020 / Published: 9 February 2020
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Alveolar epithelial type II (ATII) cells and their proper function are essential for maintaining lung integrity and homeostasis. However, they can be damaged by lipopolysaccharide (LPS) during Gram-negative bacterial infection. Thus, this study evaluated and compared the effects of LPS on short and long-term cultures of A549 cells by determining the cell viability, levels of oxidative stress and antimicrobial peptide cathelicidin LL-37 and changes in the expression of surfactant proteins (SPs). Moreover, we compared A549 cell response to LPS in the presence of different serum concentrations. Additionally, the effect of N-acetylcysteine (NAC) on LPS-induced oxidative stress as a possible treatment was determined. Our results indicate that A549 cells are relatively resistant to LPS and able to maintain integrity even at high LPS concentrations. Their response to endotoxin is partially dependent on serum concentration. NAC failed to lower LPS-induced oxidative stress in A549 cells. Finally, LPS modulates SP gene expression in A549 cells in a time dependent manner and differences between short and long-term cultures were present. Our results support the idea that long-term cultivation of A549 cells could promote a more ATII-like phenotype and thus could be a more suitable model for ATII cells, especially for in vitro studies dealing with surfactant production. View Full-Text
Keywords: acute respiratory distress syndrome; alveolar epithelial cells; bacterial lipopolysaccharide; N-acetylcysteine; cathelicidin LL-37; surfactant proteins acute respiratory distress syndrome; alveolar epithelial cells; bacterial lipopolysaccharide; N-acetylcysteine; cathelicidin LL-37; surfactant proteins
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MDPI and ACS Style

Nova, Z.; Skovierova, H.; Strnadel, J.; Halasova, E.; Calkovska, A. Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37. Int. J. Mol. Sci. 2020, 21, 1148. https://doi.org/10.3390/ijms21031148

AMA Style

Nova Z, Skovierova H, Strnadel J, Halasova E, Calkovska A. Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37. International Journal of Molecular Sciences. 2020; 21(3):1148. https://doi.org/10.3390/ijms21031148

Chicago/Turabian Style

Nova, Zuzana; Skovierova, Henrieta; Strnadel, Jan; Halasova, Erika; Calkovska, Andrea. 2020. "Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37" Int. J. Mol. Sci. 21, no. 3: 1148. https://doi.org/10.3390/ijms21031148

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