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Article

Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep

1
State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China
2
College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
3
College of Innovation Technology, Inner Mongolia University, Hohhot 010070, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(3), 1121; https://doi.org/10.3390/ijms21031121
Received: 24 December 2019 / Revised: 30 January 2020 / Accepted: 4 February 2020 / Published: 7 February 2020
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Transgenic technology has huge application potential in agriculture and medical fields, such as producing new livestock varieties with new valuable features and xenotransplantation. However, how an exogenous gene affects the host animal’s gene regulation networks and their health status is still poorly understood. In the current study, Fat-1 transgenic sheep were generated, and the tissues from 100-day abnormal (DAF_1) and normal (DAF_2) fetuses, postnatal lambs (DAF_4), transgenic-silencing (DAFG5), and -expressing (DAFG6) skin cells were collected and subjected to transcriptome sequencing, and their gene expression profiles were compared in multiple dimensions. The results were as follows. For DAF_1, its abnormal development was caused by pathogen invasion but not the introduction of the Fat-1 gene. Fat-1 expression down-regulated the genes related to the cell cycle; the NF-κB signaling pathway and the PI3K/Akt signaling pathway were down-regulated, and the PUFAs (polyunsaturated fatty acids) biosynthesis pathway was shifted toward the biosynthesis of high-level n-3 LC-PUFAs (long-chain PUFAs). Four key node genes, FADS2, PPARA, PRKACA, and ACACA, were found to be responsible for the gene expression profile shift from the Fat-1 transgenic 100-day fetus to postnatal lamb, and FADS2 may play a key role in the accumulation of n-3 LC-PUFAs in Fat-1 transgenic sheep muscle. Our study provides new insights into the FUFAs synthesis regulation in Fat-1 transgenic animals. View Full-Text
Keywords: Comparative transcriptome; Fat-1; Transgenic animal; PUFAs; FADS2 Comparative transcriptome; Fat-1; Transgenic animal; PUFAs; FADS2
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MDPI and ACS Style

Luo, R.; Zheng, Z.; Yang, C.; Zhang, X.; Cheng, L.; Su, G.; Bai, C.; Li, G. Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep. Int. J. Mol. Sci. 2020, 21, 1121. https://doi.org/10.3390/ijms21031121

AMA Style

Luo R, Zheng Z, Yang C, Zhang X, Cheng L, Su G, Bai C, Li G. Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep. International Journal of Molecular Sciences. 2020; 21(3):1121. https://doi.org/10.3390/ijms21031121

Chicago/Turabian Style

Luo, Rongsong, Zhong Zheng, Chunrong Yang, Xiaoran Zhang, Lei Cheng, Guanghua Su, Chunling Bai, and Guangpeng Li. 2020. "Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep" International Journal of Molecular Sciences 21, no. 3: 1121. https://doi.org/10.3390/ijms21031121

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