Next Article in Journal
A Role of Microtubules in Oligodendrocyte Differentiation
Next Article in Special Issue
Novel Molecular Mechanisms of Gangliosides in the Nervous System Elucidated by Genetic Engineering
Previous Article in Journal
Targeting Insulin-Like Growth Factor 1 Receptor Delays M-Phase Progression and Synergizes with Aurora B Inhibition to Suppress Cell Proliferation
Previous Article in Special Issue
GM1 Ganglioside Is A Key Factor in Maintaining the Mammalian Neuronal Functions Avoiding Neurodegeneration
Open AccessArticle

Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages

1
Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), CONICET. Universidad Nacional de Córdoba, Córdoba X5000HUA, Argentina
2
Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba X5000HUA, Argentina
*
Authors to whom correspondence should be addressed.
This paper is dedicated to the memory of Professor José Luis Daniotti, Ph.D.
Int. J. Mol. Sci. 2020, 21(3), 1063; https://doi.org/10.3390/ijms21031063
Received: 14 December 2019 / Revised: 3 February 2020 / Accepted: 3 February 2020 / Published: 5 February 2020
(This article belongs to the Special Issue Gangliosides: Modes of Action and Cell Fates)
Gangliosides are constituents of the mammalian cell membranes and participate in the inflammatory response. However, little is known about the presence and enzymatic activity of ganglioside sialyltransferases at the cell surface of macrophages, one of the most important immune cells involved in the innate inflammatory process. In the present study, using biochemical and fluorescent microscopy approaches, we found that endogenous ST8Sia-I is present at the plasma membrane (ecto-ST8Sia-I) of murine macrophage RAW264.7 cells. Moreover, ecto-ST8Sia-I can synthetize GD3 ganglioside at the cell surface in lipopolysaccharide (LPS)-stimulated macrophages even when LPS-stimulated macrophages reduced the total ST8Sia-I expression levels. Besides, cotreatment of LPS with an inhibitor of nitric oxide (NO) synthase recovered the ecto-ST8Sia-I expression, suggesting that NO production is involved in the reduction of ST8Sia-I expression. The diminution of ST8Sia-I expression in LPS-stimulated macrophages correlated with a reduction of GD3 and GM1 gangliosides and with an increment of GD1a. Taken together, the data supports the presence and activity of sialyltransferases at the plasma membrane of RAW264.7 cells. The variations of ecto-ST8Sia-I and ganglioside levels in stimulated macrophages constitutes a promissory pathway to further explore the physiological role of this and others ganglioside metabolism-related enzymes at the cell surface during the immune response. View Full-Text
Keywords: gangliosides; sialyltransferase; macrophage; cis-catalytic activity; nitric oxide; glycosphingolipids gangliosides; sialyltransferase; macrophage; cis-catalytic activity; nitric oxide; glycosphingolipids
Show Figures

Figure 1

MDPI and ACS Style

Vilcaes, A.A.; Garbarino-Pico, E.; Torres Demichelis, V.; Daniotti, J.L. Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages. Int. J. Mol. Sci. 2020, 21, 1063. https://doi.org/10.3390/ijms21031063

AMA Style

Vilcaes AA, Garbarino-Pico E, Torres Demichelis V, Daniotti JL. Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages. International Journal of Molecular Sciences. 2020; 21(3):1063. https://doi.org/10.3390/ijms21031063

Chicago/Turabian Style

Vilcaes, Aldo A.; Garbarino-Pico, Eduardo; Torres Demichelis, Vanina; Daniotti, Jose L. 2020. "Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages" Int. J. Mol. Sci. 21, no. 3: 1063. https://doi.org/10.3390/ijms21031063

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop