Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages
Abstract
:1. Introduction
2. Results
2.1. Endogenous ST8Sia-I Subcellular Localization in Macrophages
2.2. Expression and Cis-activity of Ecto-ST8Sia-I in Macrophages Stimulated by LPS
2.3. The Alteration in ST8Sia-I Expression Correlates with a Reduction of GD3 and GM1 and with an Increment of GD1a at the Plasma Membrane
2.4. Relative Levels of NEU1, NEU3 and ST3Gal-II Transcripts Analyzed by RT-qPCR
2.5. NO Formation is Involved in the Expression/Localization of ST8Sia-I at the Plasma Membrane
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Stimulation
4.2. Assay of NO Production
4.3. Antibodies
4.4. GD3 Detection by ELISA
4.5. Determination of ecto-ST8Sia-I Activity at the Cell Surface
4.6. Confocal Immunofluorescence Microscopy
4.7. Electrophoresis and Immunoblotting
4.8. Lipid Labeling, Extraction, and Chromatography
4.9. RNA Isolation and cDNA Synthesis
4.10. qPCR (Quantitative Real-time PCR)
4.11. Statistical Analyses
4.12. Image Processing
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Vilcaes, A.A.; Garbarino-Pico, E.; Torres Demichelis, V.; Daniotti, J.L. Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages. Int. J. Mol. Sci. 2020, 21, 1063. https://doi.org/10.3390/ijms21031063
Vilcaes AA, Garbarino-Pico E, Torres Demichelis V, Daniotti JL. Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages. International Journal of Molecular Sciences. 2020; 21(3):1063. https://doi.org/10.3390/ijms21031063
Chicago/Turabian StyleVilcaes, Aldo A., Eduardo Garbarino-Pico, Vanina Torres Demichelis, and Jose L. Daniotti. 2020. "Ganglioside Synthesis by Plasma Membrane-Associated Sialyltransferase in Macrophages" International Journal of Molecular Sciences 21, no. 3: 1063. https://doi.org/10.3390/ijms21031063