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Article

Caffeic and Chlorogenic Acids Synergistically Activate Browning Program in Human Adipocytes: Implications of AMPK- and PPAR-Mediated Pathways

1
Department of Plant Cell Biotechnology, Center of Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria
2
Laboratory of Metabolomics, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 4000 Plovdiv, Bulgaria
3
Department of Pharmacy, G. d’Annunzio University, 66100 Chieti, Italy
4
Division of Pediatric Endocrinology and Diabetes, Department of Pediatrics and Adolescent Medicine, Ulm University Medical Center, 89077 Ulm, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(24), 9740; https://doi.org/10.3390/ijms21249740
Received: 24 November 2020 / Revised: 16 December 2020 / Accepted: 17 December 2020 / Published: 21 December 2020
Caffeic acid (CA) and chlorogenic acid (CGA) are phenolic compounds claimed to be responsible for the metabolic effects of coffee and tea consumption. Along with their structural similarities, they share common mechanisms such as activation of the AMP-activated protein kinase (AMPK) signaling. The present study aimed to investigate the anti-obesity potential of CA and CGA as co-treatment in human adipocytes. The molecular interactions of CA and CGA with key adipogenic transcription factors were simulated through an in silico molecular docking approach. The expression levels of white and brown adipocyte markers, as well as genes related to lipid metabolism, were analyzed by real-time quantitative PCR and Western blot analyses. Mechanistically, the CA/CGA combination induced lipolysis, upregulated AMPK and browning gene expression and downregulated peroxisome proliferator-activated receptor γ (PPARγ) at both transcriptional and protein levels. The gene expression profiles of the CA/CGA-co-treated adipocytes strongly resembled brown-like signatures. Major pathways identified included the AMPK- and PPAR-related signaling pathways. Collectively, these findings indicated that CA/CGA co-stimulation exerted a browning-inducing potential superior to that of either compound used alone which merits implementation in obesity management. Further, the obtained data provide additional insights on how CA and CGA modify adipocyte function, differentiation and lipid metabolism. View Full-Text
Keywords: chlorogenic acid; caffeic acid; adipocytes; obesity; browning; anti-obesity effect; molecular docking chlorogenic acid; caffeic acid; adipocytes; obesity; browning; anti-obesity effect; molecular docking
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MDPI and ACS Style

Vasileva, L.V.; Savova, M.S.; Amirova, K.M.; Balcheva-Sivenova, Z.; Ferrante, C.; Orlando, G.; Wabitsch, M.; Georgiev, M.I. Caffeic and Chlorogenic Acids Synergistically Activate Browning Program in Human Adipocytes: Implications of AMPK- and PPAR-Mediated Pathways. Int. J. Mol. Sci. 2020, 21, 9740. https://doi.org/10.3390/ijms21249740

AMA Style

Vasileva LV, Savova MS, Amirova KM, Balcheva-Sivenova Z, Ferrante C, Orlando G, Wabitsch M, Georgiev MI. Caffeic and Chlorogenic Acids Synergistically Activate Browning Program in Human Adipocytes: Implications of AMPK- and PPAR-Mediated Pathways. International Journal of Molecular Sciences. 2020; 21(24):9740. https://doi.org/10.3390/ijms21249740

Chicago/Turabian Style

Vasileva, Liliya V., Martina S. Savova, Kristiana M. Amirova, Zhivka Balcheva-Sivenova, Claudio Ferrante, Giustino Orlando, Martin Wabitsch, and Milen I. Georgiev. 2020. "Caffeic and Chlorogenic Acids Synergistically Activate Browning Program in Human Adipocytes: Implications of AMPK- and PPAR-Mediated Pathways" International Journal of Molecular Sciences 21, no. 24: 9740. https://doi.org/10.3390/ijms21249740

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