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Open AccessArticle

Effect of Ionic Strength on Thioflavin-T Affinity to Amyloid Fibrils and Its Fluorescence Intensity

1
Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania
2
Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(23), 8916; https://doi.org/10.3390/ijms21238916
Received: 5 November 2020 / Revised: 23 November 2020 / Accepted: 24 November 2020 / Published: 24 November 2020
(This article belongs to the Special Issue The Role of Environment in Amyloid Aggregation)
The formation of amyloid fibrils is linked to multiple neurodegenerative disorders, including Alzheimer’s and Parkinson’s disease. Despite years of research and countless studies on the topic of such aggregate formation, as well as their resulting structure, the current knowledge is still fairly limited. One of the main aspects prohibiting effective aggregation tracking is the environment’s effect on amyloid-specific dyes, namely thioflavin-T (ThT). Currently, there are only a few studies hinting at ionic strength being one of the factors that modulate the dye’s binding affinity and fluorescence intensity. In this work we explore this effect under a range of ionic strength conditions, using insulin, lysozyme, mouse prion protein, and α-synuclein fibrils. We show that ionic strength is an extremely important factor affecting both the binding affinity, as well as the fluorescence intensity of ThT. View Full-Text
Keywords: amyloid; protein aggregation; ionic strength; thioflavin-T amyloid; protein aggregation; ionic strength; thioflavin-T
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MDPI and ACS Style

Mikalauskaite, K.; Ziaunys, M.; Sneideris, T.; Smirnovas, V. Effect of Ionic Strength on Thioflavin-T Affinity to Amyloid Fibrils and Its Fluorescence Intensity. Int. J. Mol. Sci. 2020, 21, 8916. https://doi.org/10.3390/ijms21238916

AMA Style

Mikalauskaite K, Ziaunys M, Sneideris T, Smirnovas V. Effect of Ionic Strength on Thioflavin-T Affinity to Amyloid Fibrils and Its Fluorescence Intensity. International Journal of Molecular Sciences. 2020; 21(23):8916. https://doi.org/10.3390/ijms21238916

Chicago/Turabian Style

Mikalauskaite, Kamile; Ziaunys, Mantas; Sneideris, Tomas; Smirnovas, Vytautas. 2020. "Effect of Ionic Strength on Thioflavin-T Affinity to Amyloid Fibrils and Its Fluorescence Intensity" Int. J. Mol. Sci. 21, no. 23: 8916. https://doi.org/10.3390/ijms21238916

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